{"id":"0edcd4f5-0d49-58bb-b575-3573144358d7","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:shmt2-loss-formyltrna","predicate":"deletion_reduces","statement":"SHMT2-null Jurkat cells failed to maintain normal mitochondrial formylmethionyl-tRNA pools.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"56b9b823-1da9-5c6c-a923-4e1031ddeb96","mechanism_event_label":"Mitochondrial serine breakdown helps prepare the tRNA that starts translation.","subject":{"id":"a5e9f537-a6cd-570d-a81e-774125a0e1e0","slug":"human-shmt2-gene","display_name":"Human SHMT2 gene","entity_type_key":"gene"},"object":{"id":"5ef1fbd8-381c-5980-95bc-b500a134092f","slug":"mitochondrial-formylmethionyl-trna","display_name":"Mitochondrial formylmethionyl-tRNA","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"56b9b823-1da9-5c6c-a923-4e1031ddeb96","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:shmt2-loss-formyltrna-event","event_type":"biochemical_relationship","label":"Mitochondrial serine breakdown helps prepare the tRNA that starts translation.","description":"SHMT2-null Jurkat cells failed to maintain normal mitochondrial formylmethionyl-tRNA pools.","status":"provisional","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"2559e210-62e5-5cd8-bc77-538a9aecd7c8","slug":"shmt2","display_name":"SHMT2","entity_type_key":"protein"},"role":"deleted enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ec73b364-a2ea-5c4a-bcea-a54aeebf57d1","slug":"10-formyltetrahydrofolate","display_name":"10-Formyltetrahydrofolate","entity_type_key":"small_molecule"},"role":"formyl donor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f6b7e980-fc6b-5c64-aa8e-9483d3d96029","slug":"mtfmt","display_name":"Human mitochondrial methionyl-tRNA formyltransferase / MTFMT","entity_type_key":"protein"},"role":"formyl-transfer enzyme","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a5e9f537-a6cd-570d-a81e-774125a0e1e0","slug":"human-shmt2-gene","display_name":"Human SHMT2 gene","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"5ef1fbd8-381c-5980-95bc-b500a134092f","slug":"mitochondrial-formylmethionyl-trna","display_name":"Mitochondrial formylmethionyl-tRNA","entity_type_key":"rna"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"CRISPR and tRNA assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Assay conditions described in the linked primary study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Several downstream mitochondrial consequences may coexist.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Mitochondrial serine breakdown helps prepare the tRNA that starts translation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[minton-2018] Serine Catabolism by SHMT2 Is Required for Proper Mitochondrial Translation Initiation and Maintenance of Formylmethionyl-tRNAs (2018). https://pubmed.ncbi.nlm.nih.gov/29452640/ DOI: 10.1016/j.molcel.2018.01.024","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Jurkat cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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Study references: [minton-2018] Serine Catabolism by SHMT2 Is Required for Proper Mitochondrial Translation Initiation and Maintenance of Formylmethionyl-tRNAs (2018). https://pubmed.ncbi.nlm.nih.gov/29452640/ DOI: 10.1016/j.molcel.2018.01.024","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}