{"id":"0e752048-e731-550e-8901-b3e4aba36c8b","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-lat1-exchange","predicate":"exchanges","statement":"Human LAT1 with SLC3A2 transported large neutral amino acids and exchanged intracellular leucine or glutamine with extracellular substrates.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"22ab2f15-40da-5d7b-9a15-4116e13e37ee","mechanism_event_label":"Shared transport depends on amino acids on both sides of the membrane.","subject":{"id":"16d078ff-692c-50eb-a34b-c61ef43821e1","slug":"slc7a5","display_name":"Human L-type amino acid transporter 1 / LAT1 / SLC7A5","entity_type_key":"protein"},"object":{"id":"6510ebff-1fd4-5b85-86cb-3266a99a3a8f","slug":"human-lat1-large-neutral-amino-acid-exchange","display_name":"Large neutral amino-acid exchange through human LAT1","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"22ab2f15-40da-5d7b-9a15-4116e13e37ee","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-lat1-exchange-event","event_type":"observed_relationship","label":"Shared transport depends on amino acids on both sides of the membrane.","description":"Human LAT1 with SLC3A2 transported large neutral amino acids and exchanged intracellular leucine or glutamine with extracellular substrates.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"16d078ff-692c-50eb-a34b-c61ef43821e1","slug":"slc7a5","display_name":"Human L-type amino acid transporter 1 / LAT1 / SLC7A5","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6510ebff-1fd4-5b85-86cb-3266a99a3a8f","slug":"human-lat1-large-neutral-amino-acid-exchange","display_name":"Large neutral amino-acid exchange through human LAT1","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c85b327e-f132-5d9a-8e4b-c2976fcc0373","slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"1ca4b4d5-616a-54d3-8244-0dc151b1bcfe","slug":"slc3a2","display_name":"4F2hc / SLC3A2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"f707c753-cc8c-5f06-90be-bb3d73c60a18","slug":"leucine","display_name":"L-Leucine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"8637bb39-2c30-5168-baaf-3e613d831db0","slug":"glutamine","display_name":"L-Glutamine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human proteins expressed in Xenopus oocytes; large-neutral-amino-acid panel.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Accessed abstract does not resolve a separate isoleucine kinetic value. Shared transport is not proof that an ordinary mixed meal causes deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Isoleucine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Shared transport depends on amino acids on both sides of the membrane.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Human L-type amino acid transporter 1 (LAT1): characterization of function and expression in tumor cell lines. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11557028/ · DOI 10.1016/s0005-2736(01)00384-4","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b6103176-51e4-5cfc-8a60-562658195f40","evidence_kind":"source_excerpt","locator":"Lines 42-48","start_line":42,"end_line":48,"excerpt":"## isoleucine-lat1-exchange\nShared transport depends on amino acids on both sides of the membrane.\nHuman LAT1 with SLC3A2 transported large neutral amino acids and exchanged intracellular leucine or glutamine with extracellular substrates.\nModel: Human proteins expressed in Xenopus oocytes; large-neutral-amino-acid panel.\nLimitations: Accessed abstract does not resolve a separate isoleucine kinetic value. Shared transport is not proof that an ordinary mixed meal causes deficiency.\nEvidence access: Primary abstract\nHuman L-type amino acid transporter 1 (LAT1): characterization of function and expression in tumor cell lines. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11557028/ · DOI 10.1016/s0005-2736(01)00384-4","model_system":"Human proteins expressed in Xenopus oocytes; large-neutral-amino-acid panel.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4b00f118-00fd-5cef-be97-13125eb958e9","stable_key":"import-09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2","title":"L-Isoleucine: transport, translation, catabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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