{"id":"0df411f7-859e-5319-ab56-e158b3a863a0","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-gdh-regulatory-variants","predicate":"reduce_gtp_control_of","statement":"Expressing a patient GLUD1 mutant in COS7 cells reproduced reduced GTP inhibition observed in the patient lymphoblasts.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f45dd205-1c18-59e3-804e-32cd6b9f7699","mechanism_event_label":"Changing the enzyme altered its response to a brake.","subject":{"id":"b877cd10-9296-5713-bb16-869ed77d4042","slug":"human-glud1-regulatory-mutations","display_name":"Human GLUD1 regulatory mutations","entity_type_key":"cellular_process"},"object":{"id":"7a2271b6-fae8-5b08-b6ae-df7a0f96919b","slug":"glud1","display_name":"GLUD1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"f45dd205-1c18-59e3-804e-32cd6b9f7699","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-gdh-regulatory-variants-event","event_type":"observed_relationship","label":"Changing the enzyme altered its response to a brake.","description":"Expressing a patient GLUD1 mutant in COS7 cells reproduced reduced GTP inhibition observed in the patient lymphoblasts.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b877cd10-9296-5713-bb16-869ed77d4042","slug":"human-glud1-regulatory-mutations","display_name":"Human GLUD1 regulatory mutations","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7a2271b6-fae8-5b08-b6ae-df7a0f96919b","slug":"glud1","display_name":"GLUD1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"c8ccc03a-29cc-5127-baf5-81eeb92ac780","slug":"gtp","display_name":"Guanosine triphosphate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"cef15a0b-c3bf-5dba-8e69-a20c118cd411","slug":"human-glud1-hyperinsulinism-hyperammonemia","display_name":"Human GLUD1 hyperinsulinism/hyperammonemia","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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