{"id":"0db25cdf-7c7f-5ae7-94dd-55dfc9e3d203","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-kupffer-tnf","predicate":"reduces_tested","statement":"Glycine reduced LPS-associated TNF-alpha production in cultured rat Kupffer cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f54a49cd-d90f-5d5a-9d2e-62cfd055b429","mechanism_event_label":"A signaling change accompanied lower inflammatory-mediator output.","subject":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"object":{"id":"4699c10e-de9c-5cf2-b95a-bfc8636c495d","slug":"rat-kupffer-tnf-output","display_name":"TNF-alpha output from LPS-stimulated rat Kupffer cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f54a49cd-d90f-5d5a-9d2e-62cfd055b429","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-kupffer-tnf-event","event_type":"observed_relationship","label":"A signaling change accompanied lower inflammatory-mediator output.","description":"Glycine reduced LPS-associated TNF-alpha production in cultured rat Kupffer cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4699c10e-de9c-5cf2-b95a-bfc8636c495d","slug":"rat-kupffer-tnf-output","display_name":"TNF-alpha output from LPS-stimulated rat Kupffer cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6d316e3b-ba0b-5161-a57e-59c2e3869810","slug":"rat-kupffer-glycine-chloride-response","display_name":"Chloride-dependent glycine response in rat Kupffer cells","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat cell-culture LPS challenge.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The cell assay does not establish a clinical effect or prove all intermediary steps.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A signaling change accompanied lower inflammatory-mediator output.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Kupffer cells contain a glycine-gated chloride channel. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9227496/ · DOI 10.1152/ajpgi.1997.272.6.G1581","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"665834c9-5872-57b5-873a-e9f7f6da2362","evidence_kind":"source_excerpt","locator":"Lines 346-352","start_line":346,"end_line":352,"excerpt":"## glycine-kupffer-tnf\nA signaling change accompanied lower inflammatory-mediator output.\nGlycine reduced LPS-associated TNF-alpha production in cultured rat Kupffer cells.\nModel: Rat cell-culture LPS challenge.\nLimitations: The cell assay does not establish a clinical effect or prove all intermediary steps.\nEvidence access: Primary abstract\nKupffer cells contain a glycine-gated chloride channel. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9227496/ · DOI 10.1152/ajpgi.1997.272.6.G1581","model_system":"Rat cell-culture LPS challenge.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"4fabd2fc8e71ca156c91023e7340be3f5a02ae03e91d3a24b231c2adfb0de0aa","revision_id":"24801e49-34a8-53c3-bdf5-b59143441450","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}