{"id":"0d42f3aa-f4e4-565e-a19c-9c449e2bc498","stable_key":"research:gpx2-reduces-peroxide","predicate":"reduces","statement":"Expressed human GPX2 exhibited glutathione-dependent hydrogen-peroxide reduction.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"negative","is_public":true,"mechanism_event_id":"e74899ba-ba2a-5fca-b31f-591468a4bb31","mechanism_event_label":"GPX2 can remove peroxide using glutathione.","subject":{"id":"23c45f6d-8ff2-5a4e-8260-6a75613a8fac","slug":"gpx2","display_name":"GPX2","entity_type_key":"protein"},"object":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"e74899ba-ba2a-5fca-b31f-591468a4bb31","stable_key":"research:gpx2-reduces-peroxide","event_type":"peroxide-reduction","label":"GPX2 can remove peroxide using glutathione.","description":"Expressed human GPX2 exhibited glutathione-dependent hydrogen-peroxide reduction.","status":"active","compartment":null,"participants":[{"entity":{"id":"23c45f6d-8ff2-5a4e-8260-6a75613a8fac","slug":"gpx2","display_name":"GPX2","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"reducing-cosubstrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"1d9b9d7b-3fdf-57af-b9dd-00194fa1de9e","slug":"oxidized-glutathione","display_name":"GSSG","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"d31225b7-8e94-5857-9ea1-67ac794b1f15","slug":"water","display_name":"H2O","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"MCF-7 transfectants","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"GPX2 cDNA expression and enzyme assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression model; not an intestinal clinical outcome.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"human","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"603a80ee-3f2a-53c3-97a4-a1671a6eac88","evidence_kind":"curated_literature_summary","locator":"lines 642-652","start_line":642,"end_line":652,"excerpt":"## gpx2-reduces-peroxide\n\nGPX2 can remove peroxide using glutathione.\n\nExpressed human GPX2 exhibited glutathione-dependent hydrogen-peroxide reduction.\n\nOrganism: human\nCell type: MCF-7 transfectants\nExperimental model: GPX2 cDNA expression and enzyme assays\nLimitations: Expression model; not an intestinal clinical outcome.\nPrimary reference: [Expression, characterization, and tissue distribution of a new cellular selenium-dependent glutathione peroxidase, GSHPx-GI](https://pubmed.ncbi.nlm.nih.gov/8428933/)","model_system":"GPX2 cDNA expression and enzyme assays","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"intestinal-redox","plain_language":"GPX2 can remove peroxide using glutathione.","evidence_scope":"direct_experimental","papers":[{"key":"chu-1993-gpx2-enzyme","title":"Expression, characterization, and tissue distribution of a new cellular selenium-dependent glutathione peroxidase, GSHPx-GI","url":"https://pubmed.ncbi.nlm.nih.gov/8428933/","doi":"10.1016/S0021-9258(18)53812-6","year":1993,"model":"Human GPX2 expressed in MCF-7 cells; tissue expression studies","summary":"Establishes enzymatic peroxide reduction and a distinctive tissue-expression pattern for GPX2."}]}}