{"id":"0d19e6b4-b693-548c-acd9-f5e9024c4984","stable_key":"00c24cfe-6189-5244-b81f-5d693e4e177d:chlorogenic_acid-ldl-initiation","predicate":"inhibits_at_initiation","statement":"CGA at 0.5 micromolar inhibited copper-induced LDL oxidation during initiation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"1b5e36cc-3217-5102-9fa7-907cc0562f6b","mechanism_event_label":"The early reaction stage showed antioxidant behavior.","subject":{"id":"705c7f84-5840-55e1-be69-383cc8793a06","slug":"chlorogenic-acid","display_name":"Chlorogenic acid / 5-O-caffeoylquinic acid","entity_type_key":"small_molecule"},"object":{"id":"51d16a59-092e-5057-ab80-6a5c1939297f","slug":"copper-ldl-oxidation","display_name":"Copper-driven LDL oxidation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"1b5e36cc-3217-5102-9fa7-907cc0562f6b","stable_key":"00c24cfe-6189-5244-b81f-5d693e4e177d:chlorogenic_acid-ldl-initiation-event","event_type":"biochemical_relationship","label":"The early reaction stage showed antioxidant behavior.","description":"CGA at 0.5 micromolar inhibited copper-induced LDL oxidation during initiation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2a870bb5-05a0-5e51-a70c-a9a843a8b571","slug":"copper-ii","display_name":"Copper(II) ion","entity_type_key":"ion"},"role":"oxidation_initiator","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"705c7f84-5840-55e1-be69-383cc8793a06","slug":"chlorogenic-acid","display_name":"Chlorogenic acid / 5-O-caffeoylquinic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"51d16a59-092e-5057-ab80-6a5c1939297f","slug":"copper-ldl-oxidation","display_name":"Copper-driven LDL oxidation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chlorogenic_acid-research/9089288.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"459e8c9d21cadd0d8e0cd76d596dd8b6deb9c373fb7d4ee9efb8e8f507ed03ff\", \"start_char\": 0, \"end_char\": 1419, \"text_sha256\": \"459e8c9d21cadd0d8e0cd76d596dd8b6deb9c373fb7d4ee9efb8e8f507ed03ff\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated human LDL oxidation with copper challenge","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"CGA 0.5 micromolar at initiation; 0.1 micromolar or more during propagation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Reaction phase and concentration determine direction. This does not show that dietary CGA plus copper produces vascular harm. 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(1997). https://pubmed.ncbi.nlm.nih.gov/9089288/ DOI: 10.1016/s0014-5793(97)00185-3","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"LDL oxidation initiation versus propagation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a3be3b75-0d11-513a-9517-349bb4fe6ed5","evidence_kind":"source_excerpt","locator":"Lines 737-748","start_line":737,"end_line":748,"excerpt":"### chlorogenic_acid-ldl-initiation\nCGA at 0.5 micromolar inhibited copper-induced LDL oxidation during initiation.\nCondition category: normal\nnutrient_topic: Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The early reaction stage showed antioxidant behavior.\norganism: Human LDL outside the body\ntissue_or_cell_type: LDL oxidation initiation versus propagation\nexperimental_model: Isolated human LDL oxidation with copper challenge\nlimitations: Reaction phase and concentration determine direction. This does not show that dietary CGA plus copper produces vascular harm. More detailed caffeic-acid timing and concentration findings must not be transferred to CGA.\nexposure: CGA 0.5 micromolar at initiation; 0.1 micromolar or more during propagation\nevidence_span: {\"source_cache\": \"artifacts/chlorogenic_acid-research/9089288.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"459e8c9d21cadd0d8e0cd76d596dd8b6deb9c373fb7d4ee9efb8e8f507ed03ff\", \"start_char\": 0, \"end_char\": 1419, \"text_sha256\": \"459e8c9d21cadd0d8e0cd76d596dd8b6deb9c373fb7d4ee9efb8e8f507ed03ff\"}\n[chlorogenic_acid-p9089288] Prooxidant activity of caffeic acid, dietary non-flavonoid phenolic acid, on Cu2+-induced low density lipoprotein oxidation. 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