{"id":"0cfe87a3-0681-5f62-b941-f36e13076928","stable_key":"research:secis2-first","predicate":"preferentially_supports","statement":"SELENOP SECIS2 preferentially supports first-UGA decoding in the tested constructs.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"positive","is_public":true,"mechanism_event_id":"b55892a2-eb61-574f-9868-3565436f35c1","mechanism_event_label":"The second SECIS element mainly helps the first selenium insertion.","subject":{"id":"191a2fa8-7c9e-5a0c-a663-550d84b35f01","slug":"selenop-secis2","display_name":"SELENOP SECIS2","entity_type_key":"rna"},"object":{"id":"c9176183-1035-58dc-b631-9961fa2b8e02","slug":"selenop-first-uga-recoding","display_name":"SELENOP first-UGA recoding","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b55892a2-eb61-574f-9868-3565436f35c1","stable_key":"research:secis2-first","event_type":"contextual_mechanism","label":"The second SECIS element mainly helps the first selenium insertion.","description":"SELENOP SECIS2 preferentially supports first-UGA decoding in the tested constructs.","status":"active","compartment":null,"participants":[{"entity":{"id":"191a2fa8-7c9e-5a0c-a663-550d84b35f01","slug":"selenop-secis2","display_name":"SELENOP SECIS2","entity_type_key":"rna"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c9176183-1035-58dc-b631-9961fa2b8e02","slug":"selenop-first-uga-recoding","display_name":"SELENOP first-UGA recoding","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"Transfected cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"SELENOP reporter SECIS/UGA mutations in mammalian cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Functional preference, not exclusive wiring or a universal rate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Zebrafish transcript in mammalian cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c37070b8-4c34-5f07-805c-a6028aae3e74","evidence_kind":"curated_literature_summary","locator":"lines 1130-1140","start_line":1130,"end_line":1140,"excerpt":"## secis2-first\n\nThe second SECIS element mainly helps the first selenium insertion.\n\nSELENOP SECIS2 preferentially supports first-UGA decoding in the tested constructs.\n\nOrganism: Zebrafish transcript in mammalian cells\nCell type: Transfected cells\nExperimental model: SELENOP reporter SECIS/UGA mutations in mammalian cells\nLimitations: Functional preference, not exclusive wiring or a universal rate.\nPrimary reference: [Efficient incorporation of multiple selenocysteines involves an inefficient decoding step serving as a potential translational checkpoint and ribosome bottleneck](https://pmc.ncbi.nlm.nih.gov/articles/PMC1698516/)","model_system":"SELENOP reporter SECIS/UGA mutations in mammalian cells","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[{"id":"b2bea10b-5f36-5f42-bec3-04d1599a1653","title":"SELENOP SECIS roles are reversed in the deep dive","kind":"contradiction","status":"corrected","why":"The source assigns first UGA to SECIS1 and later UGAs to SECIS2, opposite the experimentally observed predominant preferences.","resolution":"Preserve original and attach both qualified claims; avoid exclusive wiring language.","created_at":"2026-09-17 07:19:34","record_type":"correction","display_label":"Correction history","record_url":"/corrections/b2bea10b-5f36-5f42-bec3-04d1599a1653","literature_review":{"revision_id":"80984e03-5f0f-5877-8094-afef7637444e","start_line":1504,"end_line":1509,"papers":[{"paper_key":"stoytcheva-2006","title":"Efficient incorporation of multiple selenocysteines involves an inefficient decoding step serving as a potential translational checkpoint and ribosome bottleneck","url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC1698516/","doi":"10.1128/MCB.00856-06","year":2006,"model":"SELENOP reporter SECIS/UGA mutations in mammalian cells","summary":"SECIS2 preferentially supports first UGA; SECIS1 supports downstream processivity."}]},"sides":[{"conflict_id":"b2bea10b-5f36-5f42-bec3-04d1599a1653","ordinal":0,"label":"Original preserved statement","revision_id":"4cec6304-35d7-5560-9133-e6ea689db838","start_line":219,"end_line":219,"quote":"- **Two SECIS elements** in the 3′UTR — SECIS 1 handles UGA₁, SECIS 2 handles UGA₂₋₁₀ with much higher processivity","claim_id":null,"source_key":"corrections","source_title":"Selenium corrections and deep dive","claim_ids":[]},{"conflict_id":"b2bea10b-5f36-5f42-bec3-04d1599a1653","ordinal":1,"label":"Literature correction and experimental limits","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","start_line":1504,"end_line":1509,"quote":"## SELENOP SECIS roles are reversed in the deep dive\n\nThe source assigns first UGA to SECIS1 and later UGAs to SECIS2, opposite the experimentally observed predominant preferences.\n\nPreserve original and attach both qualified claims; avoid exclusive wiring language.\nPrimary reference: [Efficient incorporation of multiple selenocysteines involves an inefficient decoding step serving as a potential translational checkpoint and ribosome bottleneck](https://pmc.ncbi.nlm.nih.gov/articles/PMC1698516/)","claim_id":null,"source_key":"selenium-research-2026-09-17","source_title":"Selenium: literature corrections and mechanism additions","claim_ids":[]}]}],"research":{"topic":"Selenium scientific audit","plain_language":"The second SECIS element mainly helps the first selenium insertion.","evidence_scope":"direct_experimental","papers":[{"key":"stoytcheva-2006","title":"Efficient incorporation of multiple selenocysteines involves an inefficient decoding step serving as a potential translational checkpoint and ribosome bottleneck","url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC1698516/","doi":"10.1128/MCB.00856-06","year":2006,"model":"SELENOP reporter SECIS/UGA mutations in mammalian cells","summary":"SECIS2 preferentially supports first UGA; SECIS1 supports downstream processivity."}]}}