{"id":"0ce71728-ff6c-53ac-bcb4-b1158f4f16b0","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-rpe-serine","predicate":"supports","statement":"Proline supported de novo serine synthesis and reductive carboxylation in cultured human RPE.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"eda1f005-15d0-549a-b158-a453ba031595","mechanism_event_label":"Using one amino acid can support production of another.","subject":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"object":{"id":"5c3a482f-d637-5be4-8bfa-3a7efec5bc96","slug":"human-rpe-serine-synthesis","display_name":"De novo serine synthesis in human RPE","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"eda1f005-15d0-549a-b158-a453ba031595","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-rpe-serine-event","event_type":"observed_relationship","label":"Using one amino acid can support production of another.","description":"Proline supported de novo serine synthesis and reductive carboxylation in cultured human RPE.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5c3a482f-d637-5be4-8bfa-3a7efec5bc96","slug":"human-rpe-serine-synthesis","display_name":"De novo serine synthesis in human RPE","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c882cd0b-9d83-5b07-bd1c-fe4d45657dcb","slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary human RPE culture and isotope tracing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is not a direct one-step conversion or a clinical serine-repletion trial.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Proline collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Using one amino acid can support production of another.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Proline mediates metabolic communication between retinal pigment epithelial cells and the retina. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31110046/ · DOI 10.1074/jbc.RA119.007983","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f4915dc2-75b0-5748-b94c-891b00776030","evidence_kind":"source_excerpt","locator":"Lines 310-316","start_line":310,"end_line":316,"excerpt":"## l-proline-rpe-serine\nUsing one amino acid can support production of another.\nProline supported de novo serine synthesis and reductive carboxylation in cultured human RPE.\nModel: Primary human RPE culture and isotope tracing.\nLimitations: This is not a direct one-step conversion or a clinical serine-repletion trial.\nEvidence access: Primary abstract\nProline mediates metabolic communication between retinal pigment epithelial cells and the retina. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31110046/ · DOI 10.1074/jbc.RA119.007983","model_system":"Primary human RPE culture and isotope tracing.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e5aa7fc5-ee52-5376-8169-416082a89fd1","stable_key":"import-6612c190-1948-5bcf-bbe3-a7f6c50fa3cf","title":"L-Proline: synthesis, collagen processing, redox metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7b7f1981f9c7897fbb6baa19425bdc19dede78ad4d66fa554394b8337c7366fb","revision_id":"4971a925-fa70-59f8-8030-d3029a18a62f","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}