{"id":"0ccc05fd-b509-5d2e-acf9-74c06964b665","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-mars-mischarging","predicate":"increases_under_phosphorylation","statement":"Phosphorylation-mimicking MARS1 favored non-cognate tRNAs and increased methionine incorporation at normally non-methionine positions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"277d031e-9454-51ee-8dfb-0dc100393c94","mechanism_event_label":"Cells can trade some translation accuracy for a stress response.","subject":{"id":"d66988d4-32f5-5d3f-9d5e-e3035886d544","slug":"mars1","display_name":"Human cytosolic methionyl-tRNA synthetase / MARS1","entity_type_key":"protein"},"object":{"id":"36bbc5e7-86d5-52c8-9014-faf547104954","slug":"human-noncognate-methionylation","display_name":"Methionylation of non-cognate human tRNAs","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"277d031e-9454-51ee-8dfb-0dc100393c94","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-mars-mischarging-event","event_type":"observed_relationship","label":"Cells can trade some translation accuracy for a stress response.","description":"Phosphorylation-mimicking MARS1 favored non-cognate tRNAs and increased methionine incorporation at normally non-methionine positions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d66988d4-32f5-5d3f-9d5e-e3035886d544","slug":"mars1","display_name":"Human cytosolic methionyl-tRNA synthetase / MARS1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"36bbc5e7-86d5-52c8-9014-faf547104954","slug":"human-noncognate-methionylation","display_name":"Methionylation of non-cognate human tRNAs","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"c5448053-5edf-5d0f-8027-a69cc580e030","slug":"protein-methionine","display_name":"Protein-bound methionine","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human MARS1 mutants and cultured-cell/protein assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not evidence that all altered proteins retain normal function or that methionine loading is protective.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Methionine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Cells can trade some translation accuracy for a stress response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Promiscuous methionyl-tRNA synthetase mediates adaptive mistranslation to protect cells against oxidative stress. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25097229/ · DOI 10.1242/jcs.152470","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f44c106e-fa33-58ac-af85-38c22cdd239b","evidence_kind":"source_excerpt","locator":"Lines 76-82","start_line":76,"end_line":82,"excerpt":"## methionine-mars-mischarging\nCells can trade some translation accuracy for a stress response.\nPhosphorylation-mimicking MARS1 favored non-cognate tRNAs and increased methionine incorporation at normally non-methionine positions.\nModel: Human MARS1 mutants and cultured-cell/protein assays.\nLimitations: Not evidence that all altered proteins retain normal function or that methionine loading is protective.\nEvidence access: Primary full text\nPromiscuous methionyl-tRNA synthetase mediates adaptive mistranslation to protect cells against oxidative stress. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25097229/ · DOI 10.1242/jcs.152470","model_system":"Human MARS1 mutants and cultured-cell/protein assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d969b004-ccc2-5bb4-9109-8ac649137ad1","stable_key":"import-41a89233-da9a-5b7b-9c18-e19bbadcfe2d","title":"L-Methionine: transport, methylation, sulfur metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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