{"id":"0cc91d57-7cba-517e-9fbe-b0021fa9368b","stable_key":"4d8e4781-3878-5818-8d0a-7dbd6e024555:chromium-ampk-required-uptake","predicate":"required_for_protection_of","statement":"AMPK silencing abolished chromium picolinate’s protection against impaired glucose transport in the same L6 model.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2f50ba94-0806-5682-bff3-ec9a128fbe46","mechanism_event_label":"The glucose-uptake benefit also depended on AMPK in this experiment.","subject":{"id":"378e97ad-f6dc-5b66-a889-e95543049489","slug":"rat-ampk-complexes","display_name":"Rat AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"object":{"id":"f61e2e6e-d803-54f6-ab62-4ffa080c9ad6","slug":"insulin-stimulated-glucose-uptake","display_name":"Insulin-stimulated glucose uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2f50ba94-0806-5682-bff3-ec9a128fbe46","stable_key":"4d8e4781-3878-5818-8d0a-7dbd6e024555:chromium-ampk-required-uptake-event","event_type":"biochemical_relationship","label":"The glucose-uptake benefit also depended on AMPK in this experiment.","description":"AMPK silencing abolished chromium picolinate’s protection against impaired glucose transport in the same L6 model.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"89d5d39c-2663-5e0c-8eae-562a5b25a47d","slug":"chromium-iii-picolinate","display_name":"Chromium(III) picolinate / CrPic","entity_type_key":"chemical_species"},"role":"exposure","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"378e97ad-f6dc-5b66-a889-e95543049489","slug":"rat-ampk-complexes","display_name":"Rat AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f61e2e6e-d803-54f6-ab62-4ffa080c9ad6","slug":"insulin-stimulated-glucose-uptake","display_name":"Insulin-stimulated glucose uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chromium-research/24725432.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"f172a147ded27e509e27d5bcc53ad6dc3e8b22b424ab021515b205ae29bccbc4\", \"start_char\": 0, \"end_char\": 1788, \"text_sha256\": \"f172a147ded27e509e27d5bcc53ad6dc3e8b22b424ab021515b205ae29bccbc4\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Hyperinsulinemia model with AMPK silencing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Chromium picolinate during chronic low-dose insulin exposure, followed by acute insulin response; AMPK siRNA","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-culture mechanism. The indexed abstract identifies AMPK depletion without resolving the targeted catalytic isoform; family-level identity is retained. This is not a dietary repletion or human treatment experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chromium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chromium","display_name":"Chromium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rat L6 skeletal muscle myotubes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The glucose-uptake benefit also depended on AMPK in this experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chromium-p24725432] Chromium enhances insulin responsiveness via AMPK. (2014). https://pubmed.ncbi.nlm.nih.gov/24725432/ DOI: 10.1016/j.jnutbio.2014.01.007","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cultured muscle plasma membrane and cortical actin","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"573acda8-a08c-5be5-9747-89f4d62a5f15","evidence_kind":"source_excerpt","locator":"Lines 406-417","start_line":406,"end_line":417,"excerpt":"### chromium-ampk-required-uptake\nAMPK silencing abolished chromium picolinate’s protection against impaired glucose transport in the same L6 model.\nCondition category: machinery_impairment\nnutrient_topic: Chromium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The glucose-uptake benefit also depended on AMPK in this experiment.\norganism: Rat L6 skeletal muscle myotubes\ntissue_or_cell_type: Cultured muscle plasma membrane and cortical actin\nexperimental_model: Hyperinsulinemia model with AMPK silencing\nlimitations: Cell-culture mechanism. The indexed abstract identifies AMPK depletion without resolving the targeted catalytic isoform; family-level identity is retained. This is not a dietary repletion or human treatment experiment.\nexposure: Chromium picolinate during chronic low-dose insulin exposure, followed by acute insulin response; AMPK siRNA\nevidence_span: {\"source_cache\": \"artifacts/chromium-research/24725432.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"f172a147ded27e509e27d5bcc53ad6dc3e8b22b424ab021515b205ae29bccbc4\", \"start_char\": 0, \"end_char\": 1788, \"text_sha256\": \"f172a147ded27e509e27d5bcc53ad6dc3e8b22b424ab021515b205ae29bccbc4\"}\n[chromium-p24725432] Chromium enhances insulin responsiveness via AMPK. (2014). https://pubmed.ncbi.nlm.nih.gov/24725432/ DOI: 10.1016/j.jnutbio.2014.01.007","model_system":"Hyperinsulinemia model with AMPK silencing","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [chromium-p24725432] Chromium enhances insulin responsiveness via AMPK. (2014). https://pubmed.ncbi.nlm.nih.gov/24725432/ DOI: 10.1016/j.jnutbio.2014.01.007","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"fa96342a-ca01-5425-98d9-d50e29bf0abd","stable_key":"import-4d8e4781-3878-5818-8d0a-7dbd6e024555","title":"Chromium: transport, insulin signaling, nutrient interactions and essentiality debate (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"6cae22f9104c6de041b25c23b0fd2884cc57707ac0c9a87c20b0e665d16de5ab","revision_id":"22893798-8329-5295-9c9d-82169032fc79","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}