{"id":"0ca75225-d06e-5fc5-990e-8c509027b440","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-immune-resting","predicate":"induces","statement":"In unstimulated THP-1 cells, stevioside induced TNF, IL-1beta and nitric oxide release; anti-TLR4 antibody partially neutralized TNF release.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4958e433-3a27-53b2-b020-97b6704031c3","mechanism_event_label":"The same compound can stimulate resting cells while suppressing an already stimulated response.","subject":{"id":"06e03b41-c225-5fa3-8aa3-13a07a9826a9","slug":"stevioside","display_name":"Stevioside","entity_type_key":"small_molecule"},"object":{"id":"89ab8eb6-6c8b-5e11-a083-5f18f5116c8d","slug":"human-thp1-resting-cytokine-release","display_name":"Human unstimulated THP-1 cytokine release","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4958e433-3a27-53b2-b020-97b6704031c3","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-immune-resting-event","event_type":"observed_relationship","label":"The same compound can stimulate resting cells while suppressing an already stimulated response.","description":"In unstimulated THP-1 cells, stevioside induced TNF, IL-1beta and nitric oxide release; anti-TLR4 antibody partially neutralized TNF release.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"06e03b41-c225-5fa3-8aa3-13a07a9826a9","slug":"stevioside","display_name":"Stevioside","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"89ab8eb6-6c8b-5e11-a083-5f18f5116c8d","slug":"human-thp1-resting-cytokine-release","display_name":"Human unstimulated THP-1 cytokine release","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c78086c3-7657-589b-b353-1691df2d9f8f","slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"3f792f3c-3129-5995-8341-2295c5ea5b27","slug":"tlr4","display_name":"Human toll-like receptor 4 / TLR4","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"600d5b09-e092-5146-af1e-2510cf17bac6","slug":"tnf","display_name":"Tumor necrosis factor","entity_type_key":"cytokine"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"e9fa1ab4-707e-5ff9-9d60-11f503b54c58","slug":"il1b","display_name":"Interleukin-1 beta / IL1B","entity_type_key":"cytokine"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human THP-1 cells; antibody perturbation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is an explained stimulus difference, not a contradiction or proof of a clinical immune benefit.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The same compound can stimulate resting cells while suppressing an already stimulated response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Anti-Inflammatory and Immunomodulatory Activities of Stevioside and Its Metabolite Steviol on THP-1 Cells. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16448183/ · DOI 10.1021/jf0523465","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e305042f-d8e6-5354-890b-afa165474994","evidence_kind":"source_excerpt","locator":"Lines 330-336","start_line":330,"end_line":336,"excerpt":"## stevia-immune-resting\nThe same compound can stimulate resting cells while suppressing an already stimulated response.\nIn unstimulated THP-1 cells, stevioside induced TNF, IL-1beta and nitric oxide release; anti-TLR4 antibody partially neutralized TNF release.\nModel: Human THP-1 cells; antibody perturbation.\nLimitations: This is an explained stimulus difference, not a contradiction or proof of a clinical immune benefit.\nEvidence access: Primary abstract\nAnti-Inflammatory and Immunomodulatory Activities of Stevioside and Its Metabolite Steviol on THP-1 Cells. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16448183/ · DOI 10.1021/jf0523465","model_system":"Human THP-1 cells; antibody perturbation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"295304c9-609c-5b05-a18f-a00ab7b965ed","stable_key":"import-6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7","title":"Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"a36a82c2f8a0a971ea43e9e742db65f45848d13ab77310c0dd6b5cd55e31fc16","revision_id":"ea0355af-a926-511a-a7f2-1750baa95a8d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}