{"id":"0c9780bc-ada0-57d0-a027-67da4543892d","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:airway-plasmid","predicate":"decreases_in_recorded_experiment","statement":"Mucus-penetrating nanoparticle delivery of thymulin-expression plasmid after allergic disease establishment improved mouse airway inflammation, collagen/remodeling and mechanical abnormalities.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"f171d022-2933-5f2c-b7f6-80e747430c30","mechanism_event_label":"Mucus-penetrating nanoparticle delivery of thymulin-expression plasmid after allergic disease establishment improved mouse airway inflammation, collagen/remodeling and mechanical abnormalities.","subject":{"id":"657e1f4c-401e-5584-99d9-bb685bbd2bc7","slug":"thymulin-expression-plasmid","display_name":"Engineered thymulin-expression plasmid delivery","entity_type_key":"chemical_species"},"object":{"id":"0b961d61-f730-5f8d-bef3-64437745ae33","slug":"mouse-allergic-airway-disease","display_name":"Mouse allergic airway inflammation and remodeling","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f171d022-2933-5f2c-b7f6-80e747430c30","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:airway-plasmid-event","event_type":"experimental_observation","label":"Mucus-penetrating nanoparticle delivery of thymulin-expression plasmid after allergic disease establishment improved mouse airway inflammation, collagen/remodeling and mechanical abnormalities.","description":"A 2020 study delivered a thymulin-expressing plasmid using mucus-penetrating nanoparticles after allergic disease was established in mice. Airway inflammation, collagen/remodeling and mechanical abnormalities improved. BAL-fluid IL-4 and IL-13 fell, but **IL-10 protein did not significantly change**. Isolated lymphocytes showed restored IL-10 and Foxp3 transcripts; these are different endpoints from BAL-fluid protein. TGF-β and VEGF also decreased. Record the engineered expression system and delivery vehicle, not an assumed effect of an ordinary native-peptide injection. [2020 primary study](https://doi.org/10.1126/sciadv.aay7973)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"657e1f4c-401e-5584-99d9-bb685bbd2bc7","slug":"thymulin-expression-plasmid","display_name":"Engineered thymulin-expression plasmid delivery","entity_type_key":"chemical_species"},"role":"tested factor","stoichiometry":null,"state_label":"Thymulin-expression plasmid nanoparticles","sequence_order":0,"notes":""},{"entity":{"id":"0b961d61-f730-5f8d-bef3-64437745ae33","slug":"mouse-allergic-airway-disease","display_name":"Mouse allergic airway inflammation and remodeling","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Full text obtained; relevant results/methods inspected. This does not assert exhaustive figure/data reanalysis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Thymulin-expression plasmid nanoparticles\", \"comparator\": \"Allergic disease control\", \"endpoint\": \"Mucus-penetrating nanoparticle delivery of thymulin-expression plasmid after allergic disease establishment improved mouse airway inflammation, collagen/remodeling and mechanical abnormalities.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Established allergic airway disease in mice; engineered expression plus delivery vehicle, not ordinary native-peptide injection.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Mucus-penetrating nanoparticle delivery of thymulin-expression plasmid after allergic disease establishment improved mouse airway inflammation, collagen/remodeling and mechanical abnormalities.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Nanoparticle-based thymulin gene therapy therapeutically reverses key pathology of experimental allergic asthma | 2020 | DOI 10.1126/sciadv.aay7973 | PMID 32577505 | https://doi.org/10.1126/sciadv.aay7973","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 84-84; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4f85eaee-1165-5378-b757-b021e9148996","evidence_kind":"source_excerpt","locator":"Lines 84-84","start_line":84,"end_line":84,"excerpt":"A 2020 study delivered a thymulin-expressing plasmid using mucus-penetrating nanoparticles after allergic disease was established in mice. Airway inflammation, collagen/remodeling and mechanical abnormalities improved. BAL-fluid IL-4 and IL-13 fell, but **IL-10 protein did not significantly change**. Isolated lymphocytes showed restored IL-10 and Foxp3 transcripts; these are different endpoints from BAL-fluid protein. TGF-β and VEGF also decreased. Record the engineered expression system and delivery vehicle, not an assumed effect of an ordinary native-peptide injection. [2020 primary study](https://doi.org/10.1126/sciadv.aay7973)","model_system":"Established allergic airway disease in mice; engineered expression plus delivery vehicle, not ordinary native-peptide injection.","directness":"reported_statement","verification_status":"source_recorded","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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