{"id":"0c75fb18-a68f-5eb1-b085-ee4cb8356e0e","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-hlcs-inhibition","predicate":"inhibits","statement":"Resveratrol inhibited recombinant human HLCS-dependent biotinylation in vitro, with reported IC50 approximately 3.70 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"f59b2141-a3a5-5313-b4d5-671fdb0d5a37","mechanism_event_label":"A direct laboratory connection to biotin activation is searchable.","subject":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"object":{"id":"518c7af6-7e78-59ed-a9cb-3de53c9a39c8","slug":"hlcs","display_name":"Human holocarboxylase synthetase / HLCS","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"f59b2141-a3a5-5313-b4d5-671fdb0d5a37","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-hlcs-inhibition-event","event_type":"observed_relationship","label":"A direct laboratory connection to biotin activation is searchable.","description":"Resveratrol inhibited recombinant human HLCS-dependent biotinylation in vitro, with reported IC50 approximately 3.70 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"518c7af6-7e78-59ed-a9cb-3de53c9a39c8","slug":"hlcs","display_name":"Human holocarboxylase synthetase / HLCS","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Gel-based biotin-acceptor assay and dose response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Human biotin depletion or failure of biotin repletion was not demonstrated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Resveratrol collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A direct laboratory connection to biotin activation is searchable.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Resveratrol compounds inhibit human holocarboxylase synthetase and cause a lean phenotype in Drosophila melanogaster. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26303405/ · DOI 10.1016/j.jnutbio.2015.07.004","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cfd07b61-f8ba-50a0-8082-c6552b61cbc1","evidence_kind":"source_excerpt","locator":"Lines 342-348","start_line":342,"end_line":348,"excerpt":"## resveratrol-hlcs-inhibition\nA direct laboratory connection to biotin activation is searchable.\nResveratrol inhibited recombinant human HLCS-dependent biotinylation in vitro, with reported IC50 approximately 3.70 micromolar.\nModel: Gel-based biotin-acceptor assay and dose response.\nLimitations: Human biotin depletion or failure of biotin repletion was not demonstrated.\nEvidence access: Primary full text\nResveratrol compounds inhibit human holocarboxylase synthetase and cause a lean phenotype in Drosophila melanogaster. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26303405/ · DOI 10.1016/j.jnutbio.2015.07.004","model_system":"Gel-based biotin-acceptor assay and dose response.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2844eda9-a122-5357-ae47-cd58227c5994","stable_key":"import-52871722-4aa6-5453-a902-3e76356db327","title":"Resveratrol: metabolites, target selectivity and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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