{"id":"0c564160-d9eb-54a1-a91f-27b231109cd2","stable_key":"475ea55a-65e9-51c3-a539-738a6a8f683f:mangiferin-chloramphenicol-cleavage","predicate":"inhibits","statement":"The chloramphenicol treatment abolished the induced C-glucosyl-cleaving activity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b0b59d1b-ad4c-5c88-80bd-e83d317cdc11","mechanism_event_label":"Conversion requires functioning bacterial gene-expression machinery.","subject":{"id":"576e911a-8ec4-53c8-af63-6ceb519c9ba6","slug":"chloramphenicol","display_name":"Chloramphenicol","entity_type_key":"small_molecule"},"object":{"id":"f524c891-41b2-5f32-95d1-20ded064c4f7","slug":"bacteroides-mang-c-glycosyl-cleaving-activity","display_name":"Bacteroides sp. MANG inducible C-glucosyl-cleaving activity, molecular identity unresolved","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"b0b59d1b-ad4c-5c88-80bd-e83d317cdc11","stable_key":"475ea55a-65e9-51c3-a539-738a6a8f683f:mangiferin-chloramphenicol-cleavage-event","event_type":"biochemical_relationship","label":"Conversion requires functioning bacterial gene-expression machinery.","description":"The chloramphenicol treatment abolished the induced C-glucosyl-cleaving activity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"576e911a-8ec4-53c8-af63-6ceb519c9ba6","slug":"chloramphenicol","display_name":"Chloramphenicol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f524c891-41b2-5f32-95d1-20ded064c4f7","slug":"bacteroides-mang-c-glycosyl-cleaving-activity","display_name":"Bacteroides sp. MANG inducible C-glucosyl-cleaving activity, molecular identity unresolved","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/mangiferin-research/16141538.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"9bf5373ce89587337b6d0520b931f202d44771dba205220b8e340b9fa7446b88\", \"start_char\": 0, \"end_char\": 1356, \"text_sha256\": \"9bf5373ce89587337b6d0520b931f202d44771dba205220b8e340b9fa7446b88\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated anaerobic human-fecal bacterium and cell-free extracts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Mangiferin culture exposure, with transcription/translation inhibitors","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"One isolate; activity identity unresolved; not a prediction of every human microbiome.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Mangiferin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Bacteroides sp. MANG","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Conversion requires functioning bacterial gene-expression machinery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mangiferin-p16141538] Isolation of a human intestinal bacterium that transforms mangiferin to norathyriol and inducibility of the enzyme that cleaves a C-glucosyl bond. (2005). https://pubmed.ncbi.nlm.nih.gov/16141538/ DOI: 10.1248/bpb.28.1672","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Bacterial culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"8d45b83c-d18d-544f-8cdf-d2950990db76","evidence_kind":"source_excerpt","locator":"Lines 132-143","start_line":132,"end_line":143,"excerpt":"### mangiferin-chloramphenicol-cleavage\nThe chloramphenicol treatment abolished the induced C-glucosyl-cleaving activity.\nCondition category: machinery_impairment\nnutrient_topic: Mangiferin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Conversion requires functioning bacterial gene-expression machinery.\norganism: Bacteroides sp. MANG\ntissue_or_cell_type: Bacterial culture\nexperimental_model: Isolated anaerobic human-fecal bacterium and cell-free extracts\nlimitations: One isolate; activity identity unresolved; not a prediction of every human microbiome.\nexposure: Mangiferin culture exposure, with transcription/translation inhibitors\nevidence_span: {\"source_cache\": \"artifacts/mangiferin-research/16141538.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"9bf5373ce89587337b6d0520b931f202d44771dba205220b8e340b9fa7446b88\", \"start_char\": 0, \"end_char\": 1356, \"text_sha256\": \"9bf5373ce89587337b6d0520b931f202d44771dba205220b8e340b9fa7446b88\"}\n[mangiferin-p16141538] Isolation of a human intestinal bacterium that transforms mangiferin to norathyriol and inducibility of the enzyme that cleaves a C-glucosyl bond. (2005). https://pubmed.ncbi.nlm.nih.gov/16141538/ DOI: 10.1248/bpb.28.1672","model_system":"Isolated anaerobic human-fecal bacterium and cell-free extracts","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mangiferin-p16141538] Isolation of a human intestinal bacterium that transforms mangiferin to norathyriol and inducibility of the enzyme that cleaves a C-glucosyl bond. (2005). https://pubmed.ncbi.nlm.nih.gov/16141538/ DOI: 10.1248/bpb.28.1672","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"267f942a-695a-5b62-a858-f71c06321ff5","stable_key":"import-475ea55a-65e9-51c3-a539-738a6a8f683f","title":"Mangiferin: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"295cb3a13742b3caeed19347ffcbc7863f5372d983da1625a942428919b4dc98","revision_id":"d9c2ee89-ff22-5e86-9d7f-7cb7e17f9ed2","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}