{"id":"0c50f291-6856-5826-875c-f37744698c8d","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:thiamine-def-mouse-endothelial-nos3","predicate":"antagonist-assisted-depletion-increases","statement":"Thalamic microvessel endothelial NOS immunoreactivity increased after ten days of the mouse depletion protocol.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"e09b3bc6-5b87-5f78-988c-40ef060bc5cb","mechanism_event_label":"The blood-vessel lining increased its endothelial nitric oxide synthase signal.","subject":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"object":{"id":"28026033-231a-5049-9e52-5290c6974a5e","slug":"mouse-nos3","display_name":"Mouse endothelial nitric oxide synthase / Nos3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"e09b3bc6-5b87-5f78-988c-40ef060bc5cb","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:thiamine-def-mouse-endothelial-nos3-event","event_type":"observed_intervention","label":"The blood-vessel lining increased its endothelial nitric oxide synthase signal.","description":"Thalamic microvessel endothelial NOS immunoreactivity increased after ten days of the mouse depletion protocol.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f2a6b7ab-ad41-5ada-8895-41d6ce42f4e4","slug":"nitric-oxide","display_name":"NO","entity_type_key":"small_molecule"},"role":"enzyme product, not directly quantified in this observation","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"28026033-231a-5049-9e52-5290c6974a5e","slug":"mouse-nos3","display_name":"Mouse endothelial nitric oxide synthase / Nos3","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_location","value_text":"Results: NOS Induction; Figure 3","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_span","value_text":"enhanced endothelial NOS immunoreactivity","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Adult male C57BL/6 mice and Fischer 344 Brown Norway F1 rats; thiamine-deficient diet plus pyrithiamine 0.5 mg/kg/day intraperitoneally; staged brain histochemistry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Dietary thiamine removal plus daily pyrithiamine in mice or rats","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Immunostaining is not a direct nitric oxide flux measurement and does not establish a protective or damaging net effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Thiamine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The blood-vessel lining increased its endothelial nitric oxide synthase signal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[calingasan-1998-bbb-iron-nos] Induction of nitric oxide synthase and microglial responses precede selective cell death induced by chronic impairment of oxidative metabolism (1998). https://pmc.ncbi.nlm.nih.gov/articles/PMC1852979/ DOI: 10.1016/S0002-9440(10)65602-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Thalamic microvessel walls","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"87bdd006-d57e-5b29-acc2-310128a5697a","evidence_kind":"source_excerpt","locator":"Lines 1197-1209","start_line":1197,"end_line":1209,"excerpt":"### thiamine-def-mouse-endothelial-nos3\nThalamic microvessel endothelial NOS immunoreactivity increased after ten days of the mouse depletion protocol.\nCondition category: nutrient_deficiency\nnutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The blood-vessel lining increased its endothelial nitric oxide synthase signal.\norganism: Mus musculus\ntissue_or_cell_type: Thalamic microvessel walls\nexperimental_model: Adult male C57BL/6 mice and Fischer 344 Brown Norway F1 rats; thiamine-deficient diet plus pyrithiamine 0.5 mg/kg/day intraperitoneally; staged brain histochemistry.\nlimitations: Immunostaining is not a direct nitric oxide flux measurement and does not establish a protective or damaging net effect.\nevidence_location: Results: NOS Induction; Figure 3\nevidence_span: enhanced endothelial NOS immunoreactivity\nexposure: Dietary thiamine removal plus daily pyrithiamine in mice or rats\n[calingasan-1998-bbb-iron-nos] Induction of nitric oxide synthase and microglial responses precede selective cell death induced by chronic impairment of oxidative metabolism (1998). https://pmc.ncbi.nlm.nih.gov/articles/PMC1852979/ DOI: 10.1016/S0002-9440(10)65602-7","model_system":"Adult male C57BL/6 mice and Fischer 344 Brown Norway F1 rats; thiamine-deficient diet plus pyrithiamine 0.5 mg/kg/day intraperitoneally; staged brain histochemistry.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [calingasan-1998-bbb-iron-nos] Induction of nitric oxide synthase and microglial responses precede selective cell death induced by chronic impairment of oxidative metabolism (1998). https://pmc.ncbi.nlm.nih.gov/articles/PMC1852979/ DOI: 10.1016/S0002-9440(10)65602-7","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"158d2c03-ac8c-589f-8270-c468165ae346","stable_key":"import-46d15d9e-d3b5-544d-ba01-b785aa3e4f42","title":"Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f376512fb3310141315548015b387833e3af45146e73fb73cd02cee20e4ddb9c","revision_id":"53bc5eda-dec8-58cc-a56f-a9eb4ab036ef","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}