{"id":"0c0ffbb0-68e7-5107-83e7-55bf7679811b","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-tars2-editing","predicate":"hydrolyzes","statement":"Human TARS2 clears mischarged Ser-tRNA Thr during mitochondrial translation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3afd5e79-91e9-56f6-a9a4-37d564b2ce13","mechanism_event_label":"Proofreading removes serine accidentally attached to threonine tRNA.","subject":{"id":"e1304998-8f29-56cc-8535-e65d11c53582","slug":"tars2","display_name":"Human mitochondrial threonyl-tRNA synthetase / TARS2","entity_type_key":"protein"},"object":{"id":"4521d51d-220f-5953-93ef-a8b106399b36","slug":"human-mitochondrial-seryl-trna-thr","display_name":"Mischarged human mitochondrial Ser-tRNA Thr","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"3afd5e79-91e9-56f6-a9a4-37d564b2ce13","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-tars2-editing-event","event_type":"observed_relationship","label":"Proofreading removes serine accidentally attached to threonine tRNA.","description":"Human TARS2 clears mischarged Ser-tRNA Thr during mitochondrial translation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e1304998-8f29-56cc-8535-e65d11c53582","slug":"tars2","display_name":"Human mitochondrial threonyl-tRNA synthetase / TARS2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4521d51d-220f-5953-93ef-a8b106399b36","slug":"human-mitochondrial-seryl-trna-thr","display_name":"Mischarged human mitochondrial Ser-tRNA Thr","entity_type_key":"rna"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"c882cd0b-9d83-5b07-bd1c-fe4d45657dcb","slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human mitochondrial aminoacyl-tRNA synthetase biochemistry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is tRNA-bound substrate discrimination, not evidence that serine supplements cause mistranslation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Threonine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Proofreading removes serine accidentally attached to threonine tRNA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Elucidating the molecular mechanisms associated with TARS2-related mitochondrial disease. · 2022 · https://pubmed.ncbi.nlm.nih.gov/34508595/ · DOI 10.1093/hmg/ddab257","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"abf95ce3-2e84-5cd5-98dd-e1eec8c6029d","evidence_kind":"source_excerpt","locator":"Lines 74-80","start_line":74,"end_line":80,"excerpt":"## l-threonine-tars2-editing\nProofreading removes serine accidentally attached to threonine tRNA.\nHuman TARS2 clears mischarged Ser-tRNA Thr during mitochondrial translation.\nModel: Human mitochondrial aminoacyl-tRNA synthetase biochemistry.\nLimitations: This is tRNA-bound substrate discrimination, not evidence that serine supplements cause mistranslation.\nEvidence access: Primary abstract\nElucidating the molecular mechanisms associated with TARS2-related mitochondrial disease. · 2022 · https://pubmed.ncbi.nlm.nih.gov/34508595/ · DOI 10.1093/hmg/ddab257","model_system":"Human mitochondrial aminoacyl-tRNA synthetase biochemistry.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"35396730-235a-537e-8a05-c6ef2960c509","stable_key":"import-97957230-601f-5dec-8524-0812be8fadbf","title":"L-Threonine: translation, intestinal barrier, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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