{"id":"0bc6728d-484e-5c19-9507-26d34f240714","stable_key":"649e861b-265a-5912-bc36-3a73e53ef892:l-serine-serine-atp-methylation","predicate":"supports_atp_for","statement":"Serine metabolism supported the methionine cycle through de novo ATP synthesis both with and without extracellular methionine in the tested cancer cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"25bdc8a7-bee5-5e78-884f-5d265a862d98","mechanism_event_label":"Methylation needs the ATP-making side of serine metabolism as well as one-carbon donation.","subject":{"id":"c882cd0b-9d83-5b07-bd1c-fe4d45657dcb","slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"},"object":{"id":"f9784284-22a9-594e-a809-9af4abfa0e8b","slug":"human-cancer-methionine-cycle","display_name":"Methionine-cycle function in studied human cancer cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"25bdc8a7-bee5-5e78-884f-5d265a862d98","stable_key":"649e861b-265a-5912-bc36-3a73e53ef892:l-serine-serine-atp-methylation-event","event_type":"observed_relationship","label":"Methylation needs the ATP-making side of serine metabolism as well as one-carbon donation.","description":"Serine metabolism supported the methionine cycle through de novo ATP synthesis both with and without extracellular methionine in the tested cancer cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c882cd0b-9d83-5b07-bd1c-fe4d45657dcb","slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f9784284-22a9-594e-a809-9af4abfa0e8b","slug":"human-cancer-methionine-cycle","display_name":"Methionine-cycle function in studied human cancer cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cancer-cell metabolic tracing and nutrient manipulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is not proof that adding serine increases methylation in every tissue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Serine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Methylation needs the ATP-making side of serine metabolism as well as one-carbon donation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Serine Metabolism Supports the Methionine Cycle and DNA/RNA Methylation through De Novo ATP Synthesis in Cancer Cells. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26774282/ · DOI 10.1016/j.molcel.2015.12.014","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6260a105-cb79-5224-9c7a-44a795a6e5ca","evidence_kind":"source_excerpt","locator":"Lines 182-188","start_line":182,"end_line":188,"excerpt":"## l-serine-serine-atp-methylation\nMethylation needs the ATP-making side of serine metabolism as well as one-carbon donation.\nSerine metabolism supported the methionine cycle through de novo ATP synthesis both with and without extracellular methionine in the tested cancer cells.\nModel: Human cancer-cell metabolic tracing and nutrient manipulation.\nLimitations: This is not proof that adding serine increases methylation in every tissue.\nEvidence access: Primary abstract\nSerine Metabolism Supports the Methionine Cycle and DNA/RNA Methylation through De Novo ATP Synthesis in Cancer Cells. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26774282/ · DOI 10.1016/j.molcel.2015.12.014","model_system":"Human cancer-cell metabolic tracing and nutrient manipulation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"70bcf57a-36bb-563f-9073-2d616e5f155a","stable_key":"import-649e861b-265a-5912-bc36-3a73e53ef892","title":"L-Serine: synthesis, one-carbon metabolism, lipids and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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