{"id":"0b86de71-01d3-5161-81ee-033e20f9ceb8","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-fgr-knockdown","predicate":"context_dependent_observation","statement":"Fgr knockdown raised baseline macrophage phagocytosis and blunted the additional response to apigenin.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"01b78a04-7e2f-51ca-8fef-2427d7562b19","mechanism_event_label":"Fgr knockdown raised baseline macrophage phagocytosis and blunted the additional response to apigenin.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"469f4444-b348-52c2-a5b0-8d62ec73481e","slug":"mouse-apigenin-macrophage-phagocytosis","display_name":"Mouse macrophage bacterial phagocytosis in the apigenin study","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"01b78a04-7e2f-51ca-8fef-2427d7562b19","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-fgr-knockdown-event","event_type":"experimental_observation","label":"Fgr knockdown raised baseline macrophage phagocytosis and blunted the additional response to apigenin.","description":"### api-fgr-knockdown\n\nFgr knockdown raised baseline macrophage phagocytosis and blunted the additional response to apigenin.\nBaseline and incremental effects must be separated. Epistasis supports involvement without proving a single exclusive target.\nModel and exposure: Mouse macrophage knockdown; Fgr overexpression also weakened the apigenin response.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Aging-induced Alternation in the Gut Microbiota Impairs Host Antibacterial Defense. | 2025 | https://pubmed.ncbi.nlm.nih.gov/39792643/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11948050/ | https://doi.org/10.1002/advs.202411008 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Apigenin with Fgr knockdown","sequence_order":0,"notes":""},{"entity":{"id":"469f4444-b348-52c2-a5b0-8d62ec73481e","slug":"mouse-apigenin-macrophage-phagocytosis","display_name":"Mouse macrophage bacterial phagocytosis in the apigenin study","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"mixed","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"Apigenin with Fgr knockdown","comparator":"Apigenin response with control knockdown","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"knocked down","comparator":"Apigenin response with control knockdown","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"mouse-fgr","display_name":"Mouse tyrosine-protein kinase Fgr","entity_type_key":"protein"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Apigenin with Fgr knockdown\", \"comparator\": \"Apigenin response with control knockdown\", \"endpoint\": \"Fgr knockdown raised baseline macrophage phagocytosis and blunted the additional response to apigenin.\", \"effect_direction\": \"mixed\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"apigenin\", \"state\": \"Apigenin with Fgr knockdown\"}, {\"entity_slug\": \"mouse-fgr\", \"state\": \"knocked down\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Mouse macrophage knockdown; Fgr overexpression also weakened the apigenin response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Fgr knockdown raised baseline macrophage phagocytosis and blunted the additional response to apigenin.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Aging-induced Alternation in the Gut Microbiota Impairs Host Antibacterial Defense. | https://pubmed.ncbi.nlm.nih.gov/39792643/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11948050/ | https://doi.org/10.1002/advs.202411008","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-fgr-knockdown; Baseline and incremental effects must be separated. Epistasis supports involvement without proving a single exclusive target.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"4db64581-ff0d-5d4a-9475-49b9195d6d51","evidence_kind":"source_excerpt","locator":"Lines 456-462","start_line":456,"end_line":462,"excerpt":"### api-fgr-knockdown\n\nFgr knockdown raised baseline macrophage phagocytosis and blunted the additional response to apigenin.\nBaseline and incremental effects must be separated. Epistasis supports involvement without proving a single exclusive target.\nModel and exposure: Mouse macrophage knockdown; Fgr overexpression also weakened the apigenin response.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Aging-induced Alternation in the Gut Microbiota Impairs Host Antibacterial Defense. | 2025 | https://pubmed.ncbi.nlm.nih.gov/39792643/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11948050/ | https://doi.org/10.1002/advs.202411008 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"Mouse macrophage knockdown; Fgr overexpression also weakened the apigenin response.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}