{"id":"0b047322-47d2-524c-89de-0a328f678844","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-nmda-glycine","predicate":"potentiates","statement":"Glycine increased opening frequency of NMDA-activated channels in cultured mouse brain neurons, with potentiation detected at 10 nM.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ee90c9d0-686f-5135-be3b-7d62d04fd8b6","mechanism_event_label":"An amino acid usually associated with inhibition can assist an excitatory receptor.","subject":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"object":{"id":"0dfaac36-ded6-5591-bacc-aef6e58b1135","slug":"mouse-nmda-receptors","display_name":"Mouse NMDA-type receptors, subunits unresolved","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"ee90c9d0-686f-5135-be3b-7d62d04fd8b6","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-nmda-glycine-event","event_type":"observed_relationship","label":"An amino acid usually associated with inhibition can assist an excitatory receptor.","description":"Glycine increased opening frequency of NMDA-activated channels in cultured mouse brain neurons, with potentiation detected at 10 nM.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0dfaac36-ded6-5591-bacc-aef6e58b1135","slug":"mouse-nmda-receptors","display_name":"Mouse NMDA-type receptors, subunits unresolved","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse cultured neurons and outside-out patch recordings.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The effect was distinct from strychnine-sensitive glycine receptors; it does not define an oral glycine or glutamate response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An amino acid usually associated with inhibition can assist an excitatory receptor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Glycine potentiates the NMDA response in cultured mouse brain neurons. · 1987 · https://pubmed.ncbi.nlm.nih.gov/2433595/ · DOI 10.1038/325529a0","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e3db0185-e360-54ef-8ffa-c655c3253c08","evidence_kind":"source_excerpt","locator":"Lines 234-240","start_line":234,"end_line":240,"excerpt":"## glutamate-nmda-glycine\nAn amino acid usually associated with inhibition can assist an excitatory receptor.\nGlycine increased opening frequency of NMDA-activated channels in cultured mouse brain neurons, with potentiation detected at 10 nM.\nModel: Mouse cultured neurons and outside-out patch recordings.\nLimitations: The effect was distinct from strychnine-sensitive glycine receptors; it does not define an oral glycine or glutamate response.\nEvidence access: Primary abstract\nGlycine potentiates the NMDA response in cultured mouse brain neurons. · 1987 · https://pubmed.ncbi.nlm.nih.gov/2433595/ · DOI 10.1038/325529a0","model_system":"Mouse cultured neurons and outside-out patch recordings.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c6a55239-e808-5ed7-9fa3-755a18ecb29f","stable_key":"import-b7014def-74ec-599e-8347-167e24fec1f0","title":"L-Glutamate / L-glutamic acid: carbon and nitrogen allocation, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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