{"id":"0ae02f7f-0757-59cd-9ce8-30fc811c47ab","stable_key":"8600a864-6653-52c7-9718-1362c5af4577:gamma-nonalactone-hne-metabolite","predicate":"is_biotransformed_to","statement":"Gamma-nonalactone was identified among products formed during HNE biotransformation in murine astrocytes.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"0d6d9219-1631-5762-bfe1-487bd7878ad5","mechanism_event_label":"Gamma-nonalactone was identified among products formed during HNE biotransformation in murine astrocytes.","subject":{"id":"cc9bce9d-a834-5e6d-85a5-b873b8e69a21","slug":"4-hydroxynonenal","display_name":"4-Hydroxy-2-nonenal / HNE","entity_type_key":"small_molecule"},"object":{"id":"2b85fa4a-5c91-5de9-aead-a443bc4394af","slug":"gamma-nonalactone","display_name":"Gamma-nonalactone","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"0d6d9219-1631-5762-bfe1-487bd7878ad5","stable_key":"8600a864-6653-52c7-9718-1362c5af4577:gamma-nonalactone-hne-metabolite-event","event_type":"observed_relationship","label":"Gamma-nonalactone was identified among products formed during HNE biotransformation in murine astrocytes.","description":"Gamma-nonalactone was identified among products formed during HNE biotransformation in murine astrocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cc9bce9d-a834-5e6d-85a5-b873b8e69a21","slug":"4-hydroxynonenal","display_name":"4-Hydroxy-2-nonenal / HNE","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2b85fa4a-5c91-5de9-aead-a443bc4394af","slug":"gamma-nonalactone","display_name":"Gamma-nonalactone","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"trans-4-Hydroxy-2-nonenal 1, 5 or 15 micromolar","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"10 min","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract; unresolved method details explicitly retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Murine astrocyte cultures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Product identified in an HNE-metabolism study; specific formation enzymes, flux fraction and human relevance remain unresolved. The pyrrole-forming toxicity described for 4-oxo-nonanal is not a gamma-nonalactone finding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Gamma-nonalactone flavor-compound chapter; nutrient and drug interactions retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"gamma-nonalactone","display_name":"Gamma-nonalactone","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Murine astrocyte cultures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Gamma-nonalactone was identified among products formed during HNE biotransformation in murine astrocytes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Astrocytic biotransformation of trans-4-hydroxy-2-nonenal is dose-dependent. (2006). https://pubmed.ncbi.nlm.nih.gov/16780364/ DOI: 10.1021/tx0600393","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro precursor addition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"HNE biotransformation products","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1505ff16-641c-5ec5-9cce-3922d3d70dda","evidence_kind":"source_excerpt","locator":"Lines 136-145","start_line":136,"end_line":145,"excerpt":"## gamma-nonalactone-hne-metabolite\nGamma-nonalactone was identified among products formed during HNE biotransformation in murine astrocytes.\nModel/species: Murine astrocyte cultures\nTissue: HNE biotransformation products\nExposure: trans-4-Hydroxy-2-nonenal 1, 5 or 15 micromolar\nRoute: In vitro precursor addition\nDuration: 10 min\nLimits: Product identified in an HNE-metabolism study; specific formation enzymes, flux fraction and human relevance remain unresolved. The pyrrole-forming toxicity described for 4-oxo-nonanal is not a gamma-nonalactone finding.\nPrimary reference: Astrocytic biotransformation of trans-4-hydroxy-2-nonenal is dose-dependent. (2006). https://pubmed.ncbi.nlm.nih.gov/16780364/ DOI: 10.1021/tx0600393\nAccess: Primary PubMed abstract; unresolved method details explicitly retained.","model_system":"Murine astrocyte cultures","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73777c54-95a4-5b07-9d14-d4dd31949fb1","stable_key":"import-8600a864-6653-52c7-9718-1362c5af4577","title":"Gamma-nonalactone: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eight primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"a6fede6fbb2b70669be6846626f9c197edfd605c627d50fffa984e2b0b7ed71d","revision_id":"bb5ac9d2-d97d-59ac-a99c-ddf46d535574","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}