{"id":"0ab8f745-1a41-5260-aedc-945627fd52e1","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-beta-insulin","predicate":"stimulates","statement":"Results supported insulin stimulation through electrogenic transport; arginine did not directly enhance the measured exocytotic machinery.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"79207add-e89a-555b-8f51-f421d810eb43","mechanism_event_label":"A secretion effect can start at transport rather than at the final release machinery.","subject":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"object":{"id":"5e9a8b77-118f-58e4-95a0-2e6b4d8acc0d","slug":"mouse-beta-cell-insulin-secretion","display_name":"Insulin secretion from mouse beta cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"79207add-e89a-555b-8f51-f421d810eb43","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-beta-insulin-event","event_type":"observed_relationship","label":"A secretion effect can start at transport rather than at the final release machinery.","description":"Results supported insulin stimulation through electrogenic transport; arginine did not directly enhance the measured exocytotic machinery.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5e9a8b77-118f-58e4-95a0-2e6b4d8acc0d","slug":"mouse-beta-cell-insulin-secretion","display_name":"Insulin secretion from mouse beta cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6380aa3f-ac43-5f5e-b0ad-8fd7b64d68b5","slug":"mouse-beta-cell-calcium-response","display_name":"Mouse beta-cell intracellular calcium response","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d7ab69c0-cd7d-587c-b315-47af56493182","slug":"insulin","display_name":"Insulin","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse pancreatic beta cells; electrophysiology, calcium imaging and secretion experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell/animal mechanism; no universal human insulin response inferred. NAD(P)H autofluorescence did not show increased metabolism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Arginine collection; tissue, species, dose and formulation distinctions retained.","comparator":null,"unit":null,"notes":"","entity":{"slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A secretion effect can start at transport rather than at the final release machinery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Electrogenic arginine transport mediates stimulus-secretion coupling in mouse pancreatic beta-cells. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9130159/ · DOI 10.1113/jphysiol.1997.sp021955","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3435300c-7d91-5ad7-b27a-9439541fdea3","evidence_kind":"source_excerpt","locator":"Lines 318-324","start_line":318,"end_line":324,"excerpt":"## arg-beta-insulin\nA secretion effect can start at transport rather than at the final release machinery.\nResults supported insulin stimulation through electrogenic transport; arginine did not directly enhance the measured exocytotic machinery.\nModel: Mouse pancreatic beta cells; electrophysiology, calcium imaging and secretion experiments.\nLimitations: Cell/animal mechanism; no universal human insulin response inferred. NAD(P)H autofluorescence did not show increased metabolism.\nEvidence access: Primary abstract\nElectrogenic arginine transport mediates stimulus-secretion coupling in mouse pancreatic beta-cells. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9130159/ · DOI 10.1113/jphysiol.1997.sp021955","model_system":"Mouse pancreatic beta cells; electrophysiology, calcium imaging and secretion experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; primary evidence access stated per claim.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"bb9a67ea-904f-5b8a-93a4-459081212bdd","stable_key":"import-fd37d270-3395-56d6-9f1c-ddda56e606f6","title":"L-Arginine: transport, metabolic branches, nutrient interactions, availability and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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