{"id":"0aaea9d5-1bf0-56f3-9bf3-8bfcddb4817d","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-assay-tsh","predicate":"causes_false_decrease_in","statement":"The studied Vitros 5600 TSH assay showed a mean 94% decrease after biotin, with all results falling below its reference interval.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"3c9f3249-ddc2-5570-a991-30e95720a75f","mechanism_event_label":"This assay error can resemble suppressed thyroid-stimulating hormone.","subject":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"object":{"id":"2393bc12-1d3e-563a-9d3f-a8d3d108034b","slug":"vitros-tsh-assay-result","display_name":"Ortho Vitros 5600 TSH immunoassay result","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3c9f3249-ddc2-5570-a991-30e95720a75f","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-assay-tsh-event","event_type":"observed_intervention","label":"This assay error can resemble suppressed thyroid-stimulating hormone.","description":"The studied Vitros 5600 TSH assay showed a mean 94% decrease after biotin, with all results falling below its reference interval.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2393bc12-1d3e-563a-9d3f-a8d3d108034b","slug":"vitros-tsh-assay-result","display_name":"Ortho Vitros 5600 TSH immunoassay result","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/biotin-research/28973622.fulltext.txt\", \"locator\": \"Exact primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"4ee7e5947d8391b7a0fa7601d8390147b99c5ff603b3462e9d1aa7f3db42b915\", \"start_char\": 19918, \"end_char\": 20260, \"text_sha256\": \"2604315b772473fe3efb8d153000d7efcfac08a94ea918abe26153d29a429e3f\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Nonrandomized crossover study of 6 healthy adults, 37 assays and 11 analytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"10 mg oral biotin daily for 7 days","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interference depends on assay architecture and version; this study does not establish current platform performance, a universal washout time or true hormone changes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Biotin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This assay error can resemble suppressed thyroid-stimulating hormone.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b7-p28973622] Association of Biotin Ingestion With Performance of Hormone and Nonhormone Assays in Healthy Adults. 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