{"id":"0a9aba84-bb43-5411-b8c3-e13754b61946","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-gsh-adduct","predicate":"reversibly_adds_to","statement":"NO2-CLA reacted reversibly with glutathione and other low-molecular-weight thiols through electrophilic beta and delta carbons.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c7e3588d-f9e8-5485-9713-3dceaf256442","mechanism_event_label":"Glutathione can temporarily bind a CLA-derived metabolite.","subject":{"id":"806c5846-1ca4-5854-b68d-699af6b447fb","slug":"nitro-conjugated-linoleic-acid","display_name":"Nitro-conjugated linoleic acid / NO2-CLA family","entity_type_key":"chemical_species"},"object":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"c7e3588d-f9e8-5485-9713-3dceaf256442","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-gsh-adduct-event","event_type":"observed_relationship","label":"Glutathione can temporarily bind a CLA-derived metabolite.","description":"NO2-CLA reacted reversibly with glutathione and other low-molecular-weight thiols through electrophilic beta and delta carbons.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"806c5846-1ca4-5854-b68d-699af6b447fb","slug":"nitro-conjugated-linoleic-acid","display_name":"Nitro-conjugated linoleic acid / NO2-CLA family","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d2c8eb06-c13a-5e02-b49f-138535fc43ca","slug":"nitro-cla-glutathione-adducts","display_name":"Reversible NO2-CLA–glutathione adducts","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Biochemical kinetic and mass-spectrometric studies.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Adduct formation is not proof of systemic glutathione depletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Glutathione can temporarily bind a CLA-derived metabolite.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The Chemical Basis of Thiol Addition to Nitro-conjugated Linoleic Acid, a Protective Cell-signaling Lipid. · 2017 · https://pubmed.ncbi.nlm.nih.gov/27923813/ · DOI 10.1074/jbc.M116.756288","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"bee3e13b-6221-58a5-a24c-caa033b35f95","evidence_kind":"source_excerpt","locator":"Lines 342-348","start_line":342,"end_line":348,"excerpt":"## cla-gsh-adduct\nGlutathione can temporarily bind a CLA-derived metabolite.\nNO2-CLA reacted reversibly with glutathione and other low-molecular-weight thiols through electrophilic beta and delta carbons.\nModel: Biochemical kinetic and mass-spectrometric studies.\nLimitations: Adduct formation is not proof of systemic glutathione depletion.\nEvidence access: Primary abstract\nThe Chemical Basis of Thiol Addition to Nitro-conjugated Linoleic Acid, a Protective Cell-signaling Lipid. · 2017 · https://pubmed.ncbi.nlm.nih.gov/27923813/ · DOI 10.1074/jbc.M116.756288","model_system":"Biochemical kinetic and mass-spectrometric studies.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f9fe73a8a4008d52061c65abe6de7a9806da75f1c478c5adace9387b8ffaad9e","revision_id":"21196d9c-aaab-5fe1-8f32-2987c94289c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}