{"id":"0a625e97-aaf3-5fdc-aafa-44729467fbba","stable_key":"d17caac5-7018-5ca8-9e96-95afeb384bc4:pectin-fermentation-scfa","predicate":"increases","statement":"Added pectin increased SCFA generation in human fecal incubations; acetate was the major measured SCFA.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"162e0bbc-870f-5254-9566-6d24b2d611e5","mechanism_event_label":"Gut microbes can turn pectin into small organic acids.","subject":{"id":"5449346d-7614-5c4b-bbd2-03fb4dd71aeb","slug":"pectin","display_name":"Pectin, structurally heterogeneous plant polysaccharides","entity_type_key":"chemical_species"},"object":{"id":"ff98e9e9-abbe-5a76-9c93-fb9612f3aab0","slug":"fecal-culture-scfa-production","display_name":"Short-chain fatty acid production in human fecal cultures","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"162e0bbc-870f-5254-9566-6d24b2d611e5","stable_key":"d17caac5-7018-5ca8-9e96-95afeb384bc4:pectin-fermentation-scfa-event","event_type":"biochemical_relationship","label":"Gut microbes can turn pectin into small organic acids.","description":"Added pectin increased SCFA generation in human fecal incubations; acetate was the major measured SCFA.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e366e758-1fb0-5573-aed1-34f6df874ac7","slug":"acetate","display_name":"Acetate","entity_type_key":"small_molecule"},"role":"fermentation_product","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5449346d-7614-5c4b-bbd2-03fb4dd71aeb","slug":"pectin","display_name":"Pectin, structurally heterogeneous plant polysaccharides","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ff98e9e9-abbe-5a76-9c93-fb9612f3aab0","slug":"fecal-culture-scfa-production","display_name":"Short-chain fatty acid production in human fecal cultures","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/pectin-research/2317475.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"86aae760cc52aeca6f270420e20dabaffa8167770fa30c7fa8e35b1f67b7d9b4\", \"start_char\": 0, \"end_char\": 1642, \"text_sha256\": \"86aae760cc52aeca6f270420e20dabaffa8167770fa30c7fa8e35b1f67b7d9b4\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Anaerobic human fecal incubations before and after carbohydrate pre-feeding","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Pectin and comparator carbohydrates; 48-hour incubations, up to two weeks of pre-feeding","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cultures lack intestinal absorption and host metabolism; results do not quantify systemic exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pectin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pectin","display_name":"Pectin, structurally heterogeneous plant polysaccharides","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human fecal microbiota ex vivo","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Gut microbes can turn pectin into small organic acids.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[pectin-p2317475] The effect of lactulose, pectin, arabinogalactan and cellulose on the production of organic acids and metabolism of ammonia by intestinal bacteria in a faecal incubation system. (1990). https://pubmed.ncbi.nlm.nih.gov/2317475/ DOI: 10.1079/bjn19900088","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Batch cultures","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f11a3db5-8c1c-551f-a48a-44c3947afe67","evidence_kind":"source_excerpt","locator":"Lines 867-878","start_line":867,"end_line":878,"excerpt":"### pectin-fermentation-scfa\nAdded pectin increased SCFA generation in human fecal incubations; acetate was the major measured SCFA.\nCondition category: normal\nnutrient_topic: Pectin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Gut microbes can turn pectin into small organic acids.\norganism: Human fecal microbiota ex vivo\ntissue_or_cell_type: Batch cultures\nexperimental_model: Anaerobic human fecal incubations before and after carbohydrate pre-feeding\nlimitations: Cultures lack intestinal absorption and host metabolism; results do not quantify systemic exposure.\nexposure: Pectin and comparator carbohydrates; 48-hour incubations, up to two weeks of pre-feeding\nevidence_span: {\"source_cache\": \"artifacts/pectin-research/2317475.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"86aae760cc52aeca6f270420e20dabaffa8167770fa30c7fa8e35b1f67b7d9b4\", \"start_char\": 0, \"end_char\": 1642, \"text_sha256\": \"86aae760cc52aeca6f270420e20dabaffa8167770fa30c7fa8e35b1f67b7d9b4\"}\n[pectin-p2317475] The effect of lactulose, pectin, arabinogalactan and cellulose on the production of organic acids and metabolism of ammonia by intestinal bacteria in a faecal incubation system. (1990). https://pubmed.ncbi.nlm.nih.gov/2317475/ DOI: 10.1079/bjn19900088","model_system":"Anaerobic human fecal incubations before and after carbohydrate pre-feeding","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [pectin-p2317475] The effect of lactulose, pectin, arabinogalactan and cellulose on the production of organic acids and metabolism of ammonia by intestinal bacteria in a faecal incubation system. 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