{"id":"0a0d88d6-3c14-52bd-aad1-a6b08ec42cc3","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-psc-atg5","predicate":"supports","statement":"ATG5 knockdown in human pancreatic stellate cells reduced alanine concentrations in their conditioned medium.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2ef867ad-8405-5ff4-b2cb-2f78b9b75065","mechanism_event_label":"Disrupting one part of autophagy reduced alanine supplied by neighboring cells.","subject":{"id":"c0297c97-94e4-5de9-99a2-d9c57e67b346","slug":"atg5","display_name":"Human ATG5","entity_type_key":"protein"},"object":{"id":"5584b5d8-44b3-5e0c-8451-462fd5b44205","slug":"human-psc-alanine-secretion","display_name":"Alanine secretion from human pancreatic stellate cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2ef867ad-8405-5ff4-b2cb-2f78b9b75065","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-psc-atg5-event","event_type":"observed_relationship","label":"Disrupting one part of autophagy reduced alanine supplied by neighboring cells.","description":"ATG5 knockdown in human pancreatic stellate cells reduced alanine concentrations in their conditioned medium.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c0297c97-94e4-5de9-99a2-d9c57e67b346","slug":"atg5","display_name":"Human ATG5","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5584b5d8-44b3-5e0c-8451-462fd5b44205","slug":"human-psc-alanine-secretion","display_name":"Alanine secretion from human pancreatic stellate cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a5a2b182-721f-5450-a82a-f35ce9abc6b4","slug":"human-psc-autophagy","display_name":"Autophagic flux in human pancreatic stellate cells","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human PSC shRNA experiments; serum-free conditioned medium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Knockdown is machinery impairment, not low dietary alanine; residual secretion and other autophagy effects remain. Correction record: Publisher erratum corrects the second image label in Fig. 3a to hPSC-LC3 + 8988T, matching Fig. 3b; production labeling correction, not a retraction or independent study. https://www.nature.com/articles/nature19851.pdf","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Disrupting one part of autophagy reduced alanine supplied by neighboring cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Pancreatic stellate cells support tumour metabolism through autophagic alanine secretion. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27509858/ · DOI 10.1038/nature19084","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"6bad1921-5ee7-5226-825c-00fdbaf18b70","evidence_kind":"source_excerpt","locator":"Lines 144-150","start_line":144,"end_line":150,"excerpt":"## alanine-psc-atg5\nDisrupting one part of autophagy reduced alanine supplied by neighboring cells.\nATG5 knockdown in human pancreatic stellate cells reduced alanine concentrations in their conditioned medium.\nModel: Human PSC shRNA experiments; serum-free conditioned medium.\nLimitations: Knockdown is machinery impairment, not low dietary alanine; residual secretion and other autophagy effects remain. Correction record: Publisher erratum corrects the second image label in Fig. 3a to hPSC-LC3 + 8988T, matching Fig. 3b; production labeling correction, not a retraction or independent study. https://www.nature.com/articles/nature19851.pdf\nEvidence access: Primary full text\nPancreatic stellate cells support tumour metabolism through autophagic alanine secretion. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27509858/ · DOI 10.1038/nature19084","model_system":"Human PSC shRNA experiments; serum-free conditioned medium.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8ce7dd76c88b2e25fa23d93f9655a244aadb9d90f55ec8b18ecf0fd4625a61d2","revision_id":"4ed32258-0213-599a-a272-6f8f62280773","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}