{"id":"0a03f9c9-55ec-5915-87ef-5d058fa104f9","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-slc22a4-uptake","predicate":"transports","statement":"Expression of human SLC22A4 in HEK293 cells produced efficient, sodium-dependent ergothioneine uptake and intracellular retention.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"a2d56531-48de-54c7-bcf9-cdf699fa7e61","mechanism_event_label":"A specific carrier lets cells build an ergothioneine pool.","subject":{"id":"1509c925-fc5e-5a36-aa59-96bcc489965a","slug":"slc22a4","display_name":"Human ergothioneine transporter OCTN1 / SLC22A4","entity_type_key":"protein"},"object":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"a2d56531-48de-54c7-bcf9-cdf699fa7e61","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-slc22a4-uptake-event","event_type":"observed_relationship","label":"A specific carrier lets cells build an ergothioneine pool.","description":"Expression of human SLC22A4 in HEK293 cells produced efficient, sodium-dependent ergothioneine uptake and intracellular retention.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1509c925-fc5e-5a36-aa59-96bcc489965a","slug":"slc22a4","display_name":"Human ergothioneine transporter OCTN1 / SLC22A4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"1c3fdd09-5a3e-5736-8d61-6c96a39683d1","slug":"human-cellular-ergothioneine-uptake","display_name":"Human cellular ergothioneine uptake","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human transporter expressed in HEK293 cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression experiments do not quantify uptake in every human tissue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A specific carrier lets cells build an ergothioneine pool.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Discovery of the ergothioneine transporter. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15795384/ · DOI 10.1073/pnas.0408624102","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Sodium-dependent ergothioneine uptake with intracellular retention.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"Sodium-dependent ergothioneine uptake with intracellular retention.","entity":null}],"evidence":[{"id":"b14a9eff-54ff-531d-9cef-c13a8c4a26a4","evidence_kind":"source_excerpt","locator":"Lines 16-22","start_line":16,"end_line":22,"excerpt":"## ergothioneine-slc22a4-uptake\nA specific carrier lets cells build an ergothioneine pool.\nExpression of human SLC22A4 in HEK293 cells produced efficient, sodium-dependent ergothioneine uptake and intracellular retention.\nModel: Human transporter expressed in HEK293 cells.\nLimitations: Expression experiments do not quantify uptake in every human tissue.\nEvidence access: Primary abstract\nDiscovery of the ergothioneine transporter. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15795384/ · DOI 10.1073/pnas.0408624102","model_system":"Human transporter expressed in HEK293 cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b6def8119f03cfabeec2fb55ba924340dc612e1dd42f05404201fa2b7ced5259","revision_id":"f88c38e6-ffb5-5a68-83ca-4610ca2c2df4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}