{"id":"09fcf3e5-5b70-5817-958d-28f1c46e8b63","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:b12-abs-cubam-association-calcium","predicate":"not-required-for-tested-association","statement":"Human renal cubilin and AMN remained associated during coelution in EDTA, indicating that their mutual association did not require calcium under these conditions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"49077cb0-d1a3-5d69-af57-a5e9f5fe7aef","mechanism_event_label":"Calcium dependence concerns ligand binding, not every receptor interaction.","subject":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"object":{"id":"d67e8f26-bb42-5f02-af30-41f021cfd2d0","slug":"human-cubam","display_name":"Human cubilin-amnionless complex","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"49077cb0-d1a3-5d69-af57-a5e9f5fe7aef","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:b12-abs-cubam-association-calcium-event","event_type":"biochemical_relationship","label":"Calcium dependence concerns ligand binding, not every receptor interaction.","description":"Human renal cubilin and AMN remained associated during coelution in EDTA, indicating that their mutual association did not require calcium under these conditions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b6d9b937-895d-5e0a-8cc5-0454a0b7d373","slug":"vitamin-b12","display_name":"Vitamin B12 (cobalamins)","entity_type_key":"chemical_species"},"role":"nutrient","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7a3dab53-fbcd-58da-9942-21f1a3ed088d","slug":"cubn","display_name":"Human cubilin / CUBN","entity_type_key":"protein"},"role":"subunit","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bbd594ac-365b-507f-954d-20a0df38312d","slug":"amn","display_name":"Human amnionless / AMN","entity_type_key":"protein"},"role":"subunit","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"d67e8f26-bb42-5f02-af30-41f021cfd2d0","slug":"human-cubam","display_name":"Human cubilin-amnionless complex","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human kidney cubilin-AMN purification with chelation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"EDTA present during coelution","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Chelation result concerns CUBN-AMN association, not IF-cobalamin docking or whole-body calcium deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b12","display_name":"Vitamin B12 (cobalamins)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Calcium dependence concerns ligand binding, not every receptor interaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[fyfe-2004-cubam] The functional cobalamin (vitamin B12)-intrinsic factor receptor is a novel complex of cubilin and amnionless. (2004). https://pubmed.ncbi.nlm.nih.gov/14576052/ DOI: 10.1182/blood-2003-08-2852","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Renal epithelial receptor complex","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"bcea31cb-9625-580a-b52b-ef2b79f45733","evidence_kind":"source_excerpt","locator":"Lines 231-242","start_line":231,"end_line":242,"excerpt":"### b12-abs-cubam-association-calcium\nHuman renal cubilin and AMN remained associated during coelution in EDTA, indicating that their mutual association did not require calcium under these conditions.\nCondition category: normal\nnutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Calcium dependence concerns ligand binding, not every receptor interaction.\norganism: Homo sapiens\ntissue_or_cell_type: Renal epithelial receptor complex\nexperimental_model: Human kidney cubilin-AMN purification with chelation\nlimitations: Chelation result concerns CUBN-AMN association, not IF-cobalamin docking or whole-body calcium deficiency.\nexposure: EDTA present during coelution\ncross_nutrient: true\n[fyfe-2004-cubam] The functional cobalamin (vitamin B12)-intrinsic factor receptor is a novel complex of cubilin and amnionless. (2004). https://pubmed.ncbi.nlm.nih.gov/14576052/ DOI: 10.1182/blood-2003-08-2852","model_system":"Human kidney cubilin-AMN purification with chelation","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [fyfe-2004-cubam] The functional cobalamin (vitamin B12)-intrinsic factor receptor is a novel complex of cubilin and amnionless. (2004). https://pubmed.ncbi.nlm.nih.gov/14576052/ DOI: 10.1182/blood-2003-08-2852","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73145023-2982-5848-aff2-8d8875d6b9c5","stable_key":"import-7fc92b9e-9cbf-556e-8719-6b5244625250","title":"Vitamin B12: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ad5b3a51d36e856aa7fdfd1cc23f690b094689bad87d28e1a621a54675a01c92","revision_id":"118bd616-0c13-549f-bfeb-bc439715b89c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}