{"id":"09ef330b-7e9b-57dd-af8f-0140fb68f457","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-acc2-reaction","predicate":"carboxylates","statement":"Purified human ACC2 formed malonyl-CoA from acetyl-CoA in assays varying bicarbonate and ATP.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d7397c6b-7a9d-5b10-a7a3-83de2784e7d3","mechanism_event_label":"ACC2 makes the same carbon product as ACC1 but serves a distinct enzyme context.","subject":{"id":"c9808f71-628e-52db-989a-53f6f128e78c","slug":"acacb","display_name":"Human acetyl-CoA carboxylase 2 / ACACB","entity_type_key":"protein"},"object":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"d7397c6b-7a9d-5b10-a7a3-83de2784e7d3","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-acc2-reaction-event","event_type":"biochemical_relationship","label":"ACC2 makes the same carbon product as ACC1 but serves a distinct enzyme context.","description":"Purified human ACC2 formed malonyl-CoA from acetyl-CoA in assays varying bicarbonate and ATP.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"52c778e0-2b9e-5ce2-8056-a0ffdd726693","slug":"malonyl-coa","display_name":"Malonyl-CoA","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"edf44f6b-7b23-5b97-a86b-c940e5812b06","slug":"bicarbonate-ion","display_name":"Bicarbonate ion","entity_type_key":"ion"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"c9808f71-628e-52db-989a-53f6f128e78c","slug":"acacb","display_name":"Human acetyl-CoA carboxylase 2 / ACACB","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/biotin-research/19236960.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"bedd791235b10ff5942ef7177b54bca5b2243883da02fb24572691aaf5975be5\", \"start_char\": 0, \"end_char\": 896, \"text_sha256\": \"bedd791235b10ff5942ef7177b54bca5b2243883da02fb24572691aaf5975be5\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Steady-state kinetics of purified recombinant human ACC2; LC-MS/MS malonyl-CoA assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Bicarbonate, ATP, acetyl-CoA and citrate concentration matrices","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Kinetic constants are assay properties; they do not define a dietary biotin or B5 threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Biotin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"ACC2 makes the same carbon product as ACC1 but serves a distinct enzyme context.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b7-p19236960] Characterization of recombinant human acetyl-CoA carboxylase-2 steady-state kinetics. (2009). https://pubmed.ncbi.nlm.nih.gov/19236960/ DOI: 10.1016/j.bbapap.2009.02.004","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified human ACC2","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"41afc1e5-60b7-5d8c-8873-5f6b789ac970","evidence_kind":"source_excerpt","locator":"Lines 676-687","start_line":676,"end_line":687,"excerpt":"### b7-acc2-reaction\nPurified human ACC2 formed malonyl-CoA from acetyl-CoA in assays varying bicarbonate and ATP.\nCondition category: normal\nnutrient_topic: Biotin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: ACC2 makes the same carbon product as ACC1 but serves a distinct enzyme context.\norganism: Homo sapiens\ntissue_or_cell_type: Purified human ACC2\nexperimental_model: Steady-state kinetics of purified recombinant human ACC2; LC-MS/MS malonyl-CoA assay\nlimitations: Kinetic constants are assay properties; they do not define a dietary biotin or B5 threshold.\nexposure: Bicarbonate, ATP, acetyl-CoA and citrate concentration matrices\nevidence_span: {\"source_cache\": \"artifacts/biotin-research/19236960.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"bedd791235b10ff5942ef7177b54bca5b2243883da02fb24572691aaf5975be5\", \"start_char\": 0, \"end_char\": 896, \"text_sha256\": \"bedd791235b10ff5942ef7177b54bca5b2243883da02fb24572691aaf5975be5\"}\n[b7-p19236960] Characterization of recombinant human acetyl-CoA carboxylase-2 steady-state kinetics. (2009). https://pubmed.ncbi.nlm.nih.gov/19236960/ DOI: 10.1016/j.bbapap.2009.02.004","model_system":"Steady-state kinetics of purified recombinant human ACC2; LC-MS/MS malonyl-CoA assay","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b7-p19236960] Characterization of recombinant human acetyl-CoA carboxylase-2 steady-state kinetics. (2009). https://pubmed.ncbi.nlm.nih.gov/19236960/ DOI: 10.1016/j.bbapap.2009.02.004","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9608806b-adb6-5a35-b042-057147135642","stable_key":"import-08ce9896-9d1c-5bbf-b705-5bfe771091d5","title":"Biotin: carboxylases, recycling, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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