{"id":"09c9a007-02eb-5746-807f-c63a259470d3","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:renal-romk-supports-tal-salt-reabsorption","predicate":"supports","statement":"Romk-null mice had reduced, but persisting, TAL NaCl absorption by micropuncture; the companion study demonstrated loss of native apical small-conductance K channels.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"dc1c09fb-8f04-5b26-9a73-869247bde897","mechanism_event_label":"ROMK-mediated potassium recycling supports loop salt recovery, while residual salt transport survives its deletion.","subject":{"id":"bdc516b4-647c-52b3-9b85-c925a0aec2ce","slug":"kcnj1","display_name":"Renal outer medullary potassium channel / KCNJ1","entity_type_key":"protein"},"object":{"id":"564e0318-d850-5c5e-a94e-57ce730875b5","slug":"renal-sodium-reabsorption","display_name":"Renal sodium reabsorption","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"dc1c09fb-8f04-5b26-9a73-869247bde897","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:renal-romk-supports-tal-salt-reabsorption-event","event_type":"biochemical_relationship","label":"ROMK-mediated potassium recycling supports loop salt recovery, while residual salt transport survives its deletion.","description":"Romk-null mice had reduced, but persisting, TAL NaCl absorption by micropuncture; 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unverified.","entity":null}],"evidence":[{"id":"4135a7cd-817b-59f9-a15f-02eb0efd9557","evidence_kind":"source_excerpt","locator":"Lines 561-573","start_line":561,"end_line":573,"excerpt":"### renal-romk-supports-tal-salt-reabsorption\nRomk-null mice had reduced, but persisting, TAL NaCl absorption by micropuncture; the companion study demonstrated loss of native apical small-conductance K channels.\nCondition category: machinery_impairment\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: ROMK-mediated potassium recycling supports loop salt recovery, while residual salt transport survives its deletion.\norganism: Mus musculus\ntissue_or_cell_type: Thick ascending limb\nexperimental_model: Pan-Romk deletion; micropuncture and companion patch-clamp studies\nlimitations: Hydronephrosis, developmental disease and compensatory transport complicate whole-kidney endpoints.\ncross_nutrient: Loss of potassium-channel machinery impairs sodium/chloride recovery.\nevidence_location: Lorenz primary abstract: micropuncture; Lu Results: TAL patch-clamp.\n[lorenz-2002-romk-tal] Impaired renal NaCl absorption in mice lacking the ROMK potassium channel, a model for type II Bartter's syndrome (2002). https://pubmed.ncbi.nlm.nih.gov/12122007/ DOI: 10.1074/jbc.M205627200\n[lu-2002-romk-null] Absence of small conductance K+ channel (SK) activity in apical membranes of thick ascending limb and cortical collecting duct in ROMK (Bartter's) knockout mice (2002). https://pmc.ncbi.nlm.nih.gov/articles/PMC4426997/ DOI: 10.1074/jbc.M206644200","model_system":"Pan-Romk deletion; micropuncture and companion patch-clamp studies","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [lorenz-2002-romk-tal] Impaired renal NaCl absorption in mice lacking the ROMK potassium channel, a model for type II Bartter's syndrome (2002). https://pubmed.ncbi.nlm.nih.gov/12122007/ DOI: 10.1074/jbc.M205627200; [lu-2002-romk-null] Absence of small conductance K+ channel (SK) activity in apical membranes of thick ascending limb and cortical collecting duct in ROMK (Bartter's) knockout mice (2002). https://pmc.ncbi.nlm.nih.gov/articles/PMC4426997/ DOI: 10.1074/jbc.M206644200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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