{"id":"09c0c6cf-f2c1-5b15-80c3-c1ff5b4dfd8e","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-sodium-coupling-rat","predicate":"cotransports","statement":"Rat Slc22a4-mediated ergothioneine uptake was saturable, pH-sensitive and sodium-dependent, with approximately 1:1 sodium:substrate stoichiometry.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"ef5505b4-e7cb-51b2-adbd-11ade9e6654b","mechanism_event_label":"The carrier couples entry to sodium.","subject":{"id":"5b6c323d-098b-5d94-a501-3df6e4f2840e","slug":"rat-slc22a4","display_name":"Rat ergothioneine transporter Octn1 / Slc22a4","entity_type_key":"protein"},"object":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"ef5505b4-e7cb-51b2-adbd-11ade9e6654b","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-sodium-coupling-rat-event","event_type":"observed_relationship","label":"The carrier couples entry to sodium.","description":"Rat Slc22a4-mediated ergothioneine uptake was saturable, pH-sensitive and sodium-dependent, with approximately 1:1 sodium:substrate stoichiometry.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5b6c323d-098b-5d94-a501-3df6e4f2840e","slug":"rat-slc22a4","display_name":"Rat ergothioneine transporter Octn1 / Slc22a4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat transporter in HEK293 cells; uptake also examined in rat PC12 cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Rat measurements; no demonstrated benefit from increasing dietary salt.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The carrier couples entry to sodium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Functional characterization of ergothioneine transport by rat organic cation/carnitine transporter Octn1 (slc22a4). · 2008 · https://pubmed.ncbi.nlm.nih.gov/18670092/ · DOI 10.1248/bpb.31.1580","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Saturable, pH-sensitive, sodium-dependent ergothioneine uptake.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"Saturable, pH-sensitive, sodium-dependent ergothioneine uptake.","entity":null}],"evidence":[{"id":"9db3e9ce-8536-5b77-81fd-c32a2cd8fccb","evidence_kind":"source_excerpt","locator":"Lines 24-30","start_line":24,"end_line":30,"excerpt":"## ergothioneine-sodium-coupling-rat\nThe carrier couples entry to sodium.\nRat Slc22a4-mediated ergothioneine uptake was saturable, pH-sensitive and sodium-dependent, with approximately 1:1 sodium:substrate stoichiometry.\nModel: Rat transporter in HEK293 cells; uptake also examined in rat PC12 cells.\nLimitations: Rat measurements; no demonstrated benefit from increasing dietary salt.\nEvidence access: Primary abstract\nFunctional characterization of ergothioneine transport by rat organic cation/carnitine transporter Octn1 (slc22a4). · 2008 · https://pubmed.ncbi.nlm.nih.gov/18670092/ · DOI 10.1248/bpb.31.1580","model_system":"Rat transporter in HEK293 cells; uptake also examined in rat PC12 cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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