{"id":"09ace43f-b333-5d6d-8963-87ab0e1277e5","stable_key":"c953cca8-774f-596d-b27a-f13c2c0b5fce:dhc-dhc-more-cytotoxic","predicate":"reported_comparison","statement":"Dihydrocapsaicin at concentrations up to 50 micromolar did not affect cell viability while 100 and 500 micromolar led to endothelial cytotoxicity, whereas capsaicin decreased cell viability only at a concentration of 500 micromolar.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"97e9c82f-f558-52eb-a54c-b2b311df4bb5","mechanism_event_label":"On blood vessel lining cells dihydrocapsaicin turns toxic at a fifth of the concentration capsaicin needs.","subject":{"id":"6259a8e0-9611-5f60-9e1b-53bbd8eb1afd","slug":"dihydrocapsaicin","display_name":"Dihydrocapsaicin","entity_type_key":"small_molecule"},"object":{"id":"9cdd3a64-5185-5998-82a6-d25c8409b998","slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"97e9c82f-f558-52eb-a54c-b2b311df4bb5","stable_key":"c953cca8-774f-596d-b27a-f13c2c0b5fce:dhc-dhc-more-cytotoxic-event","event_type":"observed_intervention","label":"On blood vessel lining cells dihydrocapsaicin turns toxic at a fifth of the concentration capsaicin needs.","description":"Dihydrocapsaicin at concentrations up to 50 micromolar did not affect cell viability while 100 and 500 micromolar led to endothelial cytotoxicity, whereas capsaicin decreased cell viability only at a concentration of 500 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ce817df3-08db-5a4d-a9bc-03896bcdfc5a","slug":"endothelial-cell-viability","display_name":"Viability of primary human endothelial cells","entity_type_key":"cellular_process"},"role":"measured_endpoint","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6259a8e0-9611-5f60-9e1b-53bbd8eb1afd","slug":"dihydrocapsaicin","display_name":"Dihydrocapsaicin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9cdd3a64-5185-5998-82a6-d25c8409b998","slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/dihydrocapsaicin-research/36007275.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b04a74a345c83df1454aa34ad591c7cefd04325de39cde8d5118bbea005aba3b\", \"start_char\": 0, \"end_char\": 1605, \"text_sha256\": \"b04a74a345c83df1454aa34ad591c7cefd04325de39cde8d5118bbea005aba3b\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary human endothelial cells stimulated with tumour necrosis factor alpha, with viability, adhesion and radical scavenging assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Dihydrocapsaicin from below 50 up to 500 micromolar, against capsaicin and vitamin C","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The only head-to-head cytotoxicity comparison in this collection. Concentrations of 100 micromolar and above are far higher than any plasma level reported here.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Dihydrocapsaicin research collection; topical membership is not evidence of a direct clinical effect, and dihydrocapsaicin is recorded separately from capsaicin.","comparator":null,"unit":null,"notes":"","entity":{"slug":"dihydrocapsaicin","display_name":"Dihydrocapsaicin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"On blood vessel lining cells dihydrocapsaicin turns toxic at a fifth of the concentration capsaicin needs.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[dhc-p36007275] Beneficial effects of capsaicin and dihydrocapsaicin on endothelial inflammation, nitric oxide production and antioxidant activity. (2022). https://pubmed.ncbi.nlm.nih.gov/36007275/ DOI: 10.1016/j.biopha.2022.113521","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Vascular endothelium","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f6bab6ff-d467-501c-ba70-1043c95409f3","evidence_kind":"source_excerpt","locator":"Lines 660-671","start_line":660,"end_line":671,"excerpt":"### dhc-dhc-more-cytotoxic\nDihydrocapsaicin at concentrations up to 50 micromolar did not affect cell viability while 100 and 500 micromolar led to endothelial cytotoxicity, whereas capsaicin decreased cell viability only at a concentration of 500 micromolar.\nCondition category: normal\nnutrient_topic: Dihydrocapsaicin research collection; topical membership is not evidence of a direct clinical effect, and dihydrocapsaicin is recorded separately from capsaicin.\nplain_language: On blood vessel lining cells dihydrocapsaicin turns toxic at a fifth of the concentration capsaicin needs.\norganism: Human\ntissue_or_cell_type: Vascular endothelium\nexperimental_model: Primary human endothelial cells stimulated with tumour necrosis factor alpha, with viability, adhesion and radical scavenging assays\nlimitations: The only head-to-head cytotoxicity comparison in this collection. Concentrations of 100 micromolar and above are far higher than any plasma level reported here.\nexposure: Dihydrocapsaicin from below 50 up to 500 micromolar, against capsaicin and vitamin C\nevidence_span: {\"source_cache\": \"artifacts/dihydrocapsaicin-research/36007275.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b04a74a345c83df1454aa34ad591c7cefd04325de39cde8d5118bbea005aba3b\", \"start_char\": 0, \"end_char\": 1605, \"text_sha256\": \"b04a74a345c83df1454aa34ad591c7cefd04325de39cde8d5118bbea005aba3b\"}\n[dhc-p36007275] Beneficial effects of capsaicin and dihydrocapsaicin on endothelial inflammation, nitric oxide production and antioxidant activity. 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