{"id":"0995b125-4c86-57b3-b045-da853700f409","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:pro-egf-variant-loses-basolateral-signal","predicate":"reduces","statement":"Pro-EGF P1070L impaired basolateral delivery of TRPM6-stimulating EGF activity in polarized MDCK experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"f6fc667e-9a55-54f2-9da2-081c4bf293a7","mechanism_event_label":"The mutation sends insufficient active signal to the epithelial side containing the relevant receptor.","subject":{"id":"c70b8c9d-69bd-5a7d-b6ee-cba3df2f6174","slug":"pro-egf-p1070l","display_name":"Pro-EGF P1070L","entity_type_key":"protein_state"},"object":{"id":"44b74ecc-6fb5-5308-aa53-0b9272ad5e2d","slug":"basolateral-egf-release","display_name":"Basolateral EGF release","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f6fc667e-9a55-54f2-9da2-081c4bf293a7","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:pro-egf-variant-loses-basolateral-signal-event","event_type":"biochemical_relationship","label":"The mutation sends insufficient active signal to the epithelial side containing the relevant receptor.","description":"Pro-EGF P1070L impaired basolateral delivery of TRPM6-stimulating EGF activity in polarized MDCK experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ca10832e-75ea-5324-a9d9-bbdd3153c586","slug":"pro-egf","display_name":"Pro-EGF","entity_type_key":"protein"},"role":"wild-type-precursor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"844ed9d4-1202-5e1f-8db2-771dce1b0b3d","slug":"egfr","display_name":"Epidermal growth factor receptor","entity_type_key":"protein"},"role":"signal-receptor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"877a2fb0-067c-51ca-afea-f63ff16affc8","slug":"trpm6","display_name":"TRPM6","entity_type_key":"protein"},"role":"downstream-channel","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"c70b8c9d-69bd-5a7d-b6ee-cba3df2f6174","slug":"pro-egf-p1070l","display_name":"Pro-EGF P1070L","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"44b74ecc-6fb5-5308-aa53-0b9272ad5e2d","slug":"basolateral-egf-release","display_name":"Basolateral EGF release","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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