{"id":"0983a6f3-02c8-5342-8486-ccfca4f7d9fe","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-beta-calcium","predicate":"increases","statement":"The calcium response required depolarization and extracellular calcium.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"158fd18f-b221-5a29-805f-12d62cda53c1","mechanism_event_label":"The electrical change links arginine transport to calcium entry.","subject":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"object":{"id":"6380aa3f-ac43-5f5e-b0ad-8fd7b64d68b5","slug":"mouse-beta-cell-calcium-response","display_name":"Mouse beta-cell intracellular calcium response","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"158fd18f-b221-5a29-805f-12d62cda53c1","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-beta-calcium-event","event_type":"observed_relationship","label":"The electrical change links arginine transport to calcium entry.","description":"The calcium response required depolarization and extracellular calcium.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6380aa3f-ac43-5f5e-b0ad-8fd7b64d68b5","slug":"mouse-beta-cell-calcium-response","display_name":"Mouse beta-cell intracellular calcium response","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"450ebb9e-ee95-57cd-bfe1-65ee7599164f","slug":"mouse-beta-cell-depolarization","display_name":"Mouse pancreatic beta-cell membrane depolarization","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse pancreatic beta cells; electrophysiology, calcium imaging and secretion experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No direct change in whole-cell calcium or ATP-sensitive potassium currents was detected.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Arginine collection; tissue, species, dose and formulation distinctions retained.","comparator":null,"unit":null,"notes":"","entity":{"slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The electrical change links arginine transport to calcium entry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Electrogenic arginine transport mediates stimulus-secretion coupling in mouse pancreatic beta-cells. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9130159/ · DOI 10.1113/jphysiol.1997.sp021955","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3374adf8-f575-5e93-8402-4c2b477d3511","evidence_kind":"source_excerpt","locator":"Lines 310-316","start_line":310,"end_line":316,"excerpt":"## arg-beta-calcium\nThe electrical change links arginine transport to calcium entry.\nThe calcium response required depolarization and extracellular calcium.\nModel: Mouse pancreatic beta cells; electrophysiology, calcium imaging and secretion experiments.\nLimitations: No direct change in whole-cell calcium or ATP-sensitive potassium currents was detected.\nEvidence access: Primary abstract\nElectrogenic arginine transport mediates stimulus-secretion coupling in mouse pancreatic beta-cells. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9130159/ · DOI 10.1113/jphysiol.1997.sp021955","model_system":"Mouse pancreatic beta cells; electrophysiology, calcium imaging and secretion experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; primary evidence access stated per claim.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"bb9a67ea-904f-5b8a-93a4-459081212bdd","stable_key":"import-fd37d270-3395-56d6-9f1c-ddda56e606f6","title":"L-Arginine: transport, metabolic branches, nutrient interactions, availability and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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