{"id":"093110ac-4fc8-56de-a63e-71e9960196f2","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-rat-cysteine-reversal","predicate":"reverses_tested","statement":"Adding 0.5% cysteine for twelve weeks largely reversed the low-adiposity and related metabolic phenotype of methionine restriction without restoring serum methionine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6102d5b4-0d17-56f5-b14a-ad164c33d591","mechanism_event_label":"Changing the downstream sulfur supply changed the dietary response.","subject":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"object":{"id":"71b1a819-3d6d-5574-92fa-263c261cfc4a","slug":"rat-methionine-restriction-adiposity","display_name":"Adiposity and metabolic responses to methionine restriction in rats","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6102d5b4-0d17-56f5-b14a-ad164c33d591","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-rat-cysteine-reversal-event","event_type":"observed_relationship","label":"Changing the downstream sulfur supply changed the dietary response.","description":"Adding 0.5% cysteine for twelve weeks largely reversed the low-adiposity and related metabolic phenotype of methionine restriction without restoring serum methionine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"71b1a819-3d6d-5574-92fa-263c261cfc4a","slug":"rat-methionine-restriction-adiposity","display_name":"Adiposity and metabolic responses to methionine restriction in rats","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2fa490e6-50cc-5a22-b1ea-af5e128666e4","slug":"experimental-methionine-restriction","display_name":"Experimentally methionine-restricted diet","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Four-group rat feeding study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not imply all restriction effects are exclusively due to cysteine in every species.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Methionine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Changing the downstream sulfur supply changed the dietary response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cysteine supplementation reverses methionine restriction effects on rat adiposity: significance of stearoyl-coenzyme A desaturase. · 2011 · https://pubmed.ncbi.nlm.nih.gov/20871132/ · DOI 10.1194/jlr.M010215","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ce405deb-fac3-5c54-8b4f-1db26838e2df","evidence_kind":"source_excerpt","locator":"Lines 364-370","start_line":364,"end_line":370,"excerpt":"## methionine-rat-cysteine-reversal\nChanging the downstream sulfur supply changed the dietary response.\nAdding 0.5% cysteine for twelve weeks largely reversed the low-adiposity and related metabolic phenotype of methionine restriction without restoring serum methionine.\nModel: Four-group rat feeding study.\nLimitations: Does not imply all restriction effects are exclusively due to cysteine in every species.\nEvidence access: Primary abstract\nCysteine supplementation reverses methionine restriction effects on rat adiposity: significance of stearoyl-coenzyme A desaturase. · 2011 · https://pubmed.ncbi.nlm.nih.gov/20871132/ · DOI 10.1194/jlr.M010215","model_system":"Four-group rat feeding study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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