{"id":"09231edb-9ca8-5747-be71-7688a3cac17f","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-gpr158-camp","predicate":"mediates_tested","statement":"Added glycine reduced cAMP in GPR158-expressing U87 and HEK293 assays; coexpression of RGS7/G-beta5 strengthened the response.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"faa9208e-a8bf-50e2-9402-ebd38ad0f888","mechanism_event_label":"A receptor connects glycine to an intracellular messenger.","subject":{"id":"c1793375-e328-5a81-940b-046c14ad45fe","slug":"gpr158-recombinant-assay","display_name":"Recombinant GPR158 in the 2023 binding/signaling assays","entity_type_key":"protein"},"object":{"id":"9d632169-6f2f-5bd2-bb17-e5c59c440625","slug":"gpr158-assay-camp-reduction","display_name":"cAMP reduction in GPR158-expressing cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"faa9208e-a8bf-50e2-9402-ebd38ad0f888","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-gpr158-camp-event","event_type":"observed_relationship","label":"A receptor connects glycine to an intracellular messenger.","description":"Added glycine reduced cAMP in GPR158-expressing U87 and HEK293 assays; coexpression of RGS7/G-beta5 strengthened the response.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c1793375-e328-5a81-940b-046c14ad45fe","slug":"gpr158-recombinant-assay","display_name":"Recombinant GPR158 in the 2023 binding/signaling assays","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9d632169-6f2f-5bd2-bb17-e5c59c440625","slug":"gpr158-assay-camp-reduction","display_name":"cAMP reduction in GPR158-expressing cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ad57ab75-a572-5b0f-b6a9-0374f57d4a9d","slug":"gpr158-rgs7-gnb5-assay","display_name":"GPR158-associated RGS7/G-beta5 assay complex","entity_type_key":"protein_complex"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant expression and cAMP biosensors.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Direction of cAMP regulation is cell-context dependent; do not infer universal cAMP lowering.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A receptor connects glycine to an intracellular messenger.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Orphan receptor GPR158 serves as a metabotropic glycine receptor: mGlyR. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36996198/ · DOI 10.1126/science.add7150","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"88a57681-417f-552e-82b6-dd63e4b7496b","evidence_kind":"source_excerpt","locator":"Lines 234-240","start_line":234,"end_line":240,"excerpt":"## glycine-gpr158-camp\nA receptor connects glycine to an intracellular messenger.\nAdded glycine reduced cAMP in GPR158-expressing U87 and HEK293 assays; coexpression of RGS7/G-beta5 strengthened the response.\nModel: Recombinant expression and cAMP biosensors.\nLimitations: Direction of cAMP regulation is cell-context dependent; do not infer universal cAMP lowering.\nEvidence access: Primary full text\nOrphan receptor GPR158 serves as a metabotropic glycine receptor: mGlyR. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36996198/ · DOI 10.1126/science.add7150","model_system":"Recombinant expression and cAMP biosensors.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"4fabd2fc8e71ca156c91023e7340be3f5a02ae03e91d3a24b231c2adfb0de0aa","revision_id":"24801e49-34a8-53c3-bdf5-b59143441450","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}