{"id":"0921f6a6-ac9d-52ff-91d8-6aa3b753722d","stable_key":"e13b03e1-a614-543a-ac1b-97df18cfe30d:iron-mitoferrin-fech-complex","predicate":"associates_with","statement":"Fech interacted with both Mfrn1 and Abcb10 in the studied erythroid systems.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f4cf41bc-cd36-579b-bccb-352d147fd734","mechanism_event_label":"Iron import and the final heme-synthesis enzyme can be physically organized together.","subject":{"id":"814a6cbb-8850-551e-b680-9e2398dfd61d","slug":"mouse-fech","display_name":"Mouse ferrochelatase / Fech","entity_type_key":"protein"},"object":{"id":"dccf941b-8faf-5cca-b531-b3f08f30804a","slug":"mouse-slc25a37","display_name":"Mouse mitoferrin-1 / Slc25a37","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"f4cf41bc-cd36-579b-bccb-352d147fd734","stable_key":"e13b03e1-a614-543a-ac1b-97df18cfe30d:iron-mitoferrin-fech-complex-event","event_type":"biochemical_relationship","label":"Iron import and the final heme-synthesis enzyme can be physically organized together.","description":"Fech interacted with both Mfrn1 and Abcb10 in the studied erythroid systems.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3e952ec5-d906-5b38-9ba2-ab9028e678b6","slug":"mouse-abcb10","display_name":"Mouse mitochondrial ABC transporter / Abcb10","entity_type_key":"protein"},"role":"interacting_partner","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"814a6cbb-8850-551e-b680-9e2398dfd61d","slug":"mouse-fech","display_name":"Mouse ferrochelatase / Fech","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dccf941b-8faf-5cca-b531-b3f08f30804a","slug":"mouse-slc25a37","display_name":"Mouse mitoferrin-1 / Slc25a37","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/iron-research/20427704.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a4f3b1c37151557d4d888126ba888dd95391254c5e40d1c3576f0520eed7517c\", \"start_char\": 0, \"end_char\": 1059, \"text_sha256\": \"a4f3b1c37151557d4d888126ba888dd95391254c5e40d1c3576f0520eed7517c\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Affinity purification and immunoprecipitation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Mfrn1, Abcb10 and Fech interaction analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Physical association supports coordination; direct substrate channeling was not separately proven.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse erythroleukemia cells and heterologous HEK293 expression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Iron import and the final heme-synthesis enzyme can be physically organized together.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[iron-p20427704] Ferrochelatase forms an oligomeric complex with mitoferrin-1 and Abcb10 for erythroid heme biosynthesis. (2010). https://pubmed.ncbi.nlm.nih.gov/20427704/ DOI: 10.1182/blood-2009-12-259614","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Mitochondrial heme-synthesis machinery","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"75c7c75a-cb83-5b47-9276-74b5be87ac2a","evidence_kind":"source_excerpt","locator":"Lines 498-509","start_line":498,"end_line":509,"excerpt":"### iron-mitoferrin-fech-complex\nFech interacted with both Mfrn1 and Abcb10 in the studied erythroid systems.\nCondition category: normal\nnutrient_topic: Iron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Iron import and the final heme-synthesis enzyme can be physically organized together.\norganism: Mouse erythroleukemia cells and heterologous HEK293 expression\ntissue_or_cell_type: Mitochondrial heme-synthesis machinery\nexperimental_model: Affinity purification and immunoprecipitation\nlimitations: Physical association supports coordination; direct substrate channeling was not separately proven.\nexposure: Mfrn1, Abcb10 and Fech interaction analysis\nevidence_span: {\"source_cache\": \"artifacts/iron-research/20427704.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a4f3b1c37151557d4d888126ba888dd95391254c5e40d1c3576f0520eed7517c\", \"start_char\": 0, \"end_char\": 1059, \"text_sha256\": \"a4f3b1c37151557d4d888126ba888dd95391254c5e40d1c3576f0520eed7517c\"}\n[iron-p20427704] Ferrochelatase forms an oligomeric complex with mitoferrin-1 and Abcb10 for erythroid heme biosynthesis. 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