{"id":"08974637-961c-5603-ac74-dda61983d111","stable_key":"a8bedee0-a740-5bbb-921e-bfd144c7b68c:astaxanthin-intestinal-inflammatory-signal","predicate":"reduces","statement":"All-E, 9Z and 13Z astaxanthin at 1.2 micromolar reduced TNF-induced IL-8 secretion by 22–27% in Caco-2 cells, with reduced I-kappa-B-alpha phosphorylation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5f6407bb-c977-5748-aa87-f7a86384802e","mechanism_event_label":"The absorbed forms also changed inflammatory signaling in intestinal cells.","subject":{"id":"1bf8b9c0-9c38-5c8a-b5da-f00b0eeca36f","slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"},"object":{"id":"5dd031e8-9ede-5db7-a4e1-961de31f0b49","slug":"cxcl8","display_name":"Human interleukin-8 / CXCL8","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"5f6407bb-c977-5748-aa87-f7a86384802e","stable_key":"a8bedee0-a740-5bbb-921e-bfd144c7b68c:astaxanthin-intestinal-inflammatory-signal-event","event_type":"observed_relationship","label":"The absorbed forms also changed inflammatory signaling in intestinal cells.","description":"All-E, 9Z and 13Z astaxanthin at 1.2 micromolar reduced TNF-induced IL-8 secretion by 22–27% in Caco-2 cells, with reduced I-kappa-B-alpha phosphorylation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1bf8b9c0-9c38-5c8a-b5da-f00b0eeca36f","slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5dd031e8-9ede-5db7-a4e1-961de31f0b49","slug":"cxcl8","display_name":"Human interleukin-8 / CXCL8","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e5a53562-0048-521a-9314-e157c7225fd3","slug":"all-e-astaxanthin","display_name":"All-E astaxanthin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"65ec3f99-1845-5f37-bcc7-5b68912ac772","slug":"9z-astaxanthin","display_name":"9Z astaxanthin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"be0b148a-7020-59b8-9f1c-c6c30492929c","slug":"13z-astaxanthin","display_name":"13Z astaxanthin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"4b7e6069-53d4-58cf-be3c-d63e362025bf","slug":"nfkbia","display_name":"Human I-kappa-B alpha / NFKBIA","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human Caco-2 monolayers with TNF challenge.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Different isomer responses do not establish a preferred clinical formulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Astaxanthin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The absorbed forms also changed inflammatory signaling in intestinal cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Anti-Inflammatory Effects of Different Astaxanthin Isomers and the Roles of Lipid Transporters in the Cellular Transport of Astaxanthin Isomers in Caco-2 Cell Monolayers. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31117505/ · DOI 10.1021/acs.jafc.9b02102","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"40787cc8-0219-5efd-aaba-f5d9ecbb54cb","evidence_kind":"source_excerpt","locator":"Lines 486-492","start_line":486,"end_line":492,"excerpt":"## astaxanthin-intestinal-inflammatory-signal\nThe absorbed forms also changed inflammatory signaling in intestinal cells.\nAll-E, 9Z and 13Z astaxanthin at 1.2 micromolar reduced TNF-induced IL-8 secretion by 22–27% in Caco-2 cells, with reduced I-kappa-B-alpha phosphorylation.\nModel: Human Caco-2 monolayers with TNF challenge.\nLimitations: Different isomer responses do not establish a preferred clinical formulation.\nEvidence access: Primary abstract\nAnti-Inflammatory Effects of Different Astaxanthin Isomers and the Roles of Lipid Transporters in the Cellular Transport of Astaxanthin Isomers in Caco-2 Cell Monolayers. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31117505/ · DOI 10.1021/acs.jafc.9b02102","model_system":"Human Caco-2 monolayers with TNF challenge.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4fd91ea6-78b4-5bfe-93e3-686fbef9f678","stable_key":"import-a8bedee0-a740-5bbb-921e-bfd144c7b68c","title":"Astaxanthin: transport, membrane chemistry, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"98640e32f62e6c0387578165342ad21b3d2325701fcfdbb5c48886c783040e5e","revision_id":"a82757ce-1feb-582c-a5fc-f7de36308f1f","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}