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alternating 20 Hz and 0.2 Hz stimulation and naturalistic patterns.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is a local experimental supply limitation, not a dietary glutamate-deficiency threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A local transmitter supply can become insufficient when demand rises.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A local glutamate-glutamine cycle sustains synaptic excitatory transmitter release. · 2014 · https://pubmed.ncbi.nlm.nih.gov/24559677/ · DOI 10.1016/j.neuron.2013.12.026","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2093e036-48d4-5a76-8fba-269eac7cc542","evidence_kind":"source_excerpt","locator":"Lines 130-136","start_line":130,"end_line":136,"excerpt":"## glutamate-recycling-demand\nA local transmitter supply can become insufficient when demand rises.\nRepeated high-frequency stimulation of rat hippocampal and cortical terminals reduced synaptic efficacy and glutamate release; the effect depended on activity pattern.\nModel: Rat brain slices with axons transected; alternating 20 Hz and 0.2 Hz stimulation and naturalistic patterns.\nLimitations: This is a local experimental supply limitation, not a dietary glutamate-deficiency threshold.\nEvidence access: Primary full text\nA local glutamate-glutamine cycle sustains synaptic excitatory transmitter release. · 2014 · https://pubmed.ncbi.nlm.nih.gov/24559677/ · DOI 10.1016/j.neuron.2013.12.026","model_system":"Rat brain slices with axons transected; alternating 20 Hz and 0.2 Hz stimulation and naturalistic patterns.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c6a55239-e808-5ed7-9fa3-755a18ecb29f","stable_key":"import-b7014def-74ec-599e-8347-167e24fec1f0","title":"L-Glutamate / L-glutamic acid: carbon and nitrogen allocation, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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