{"id":"080ec7eb-36cd-5729-be8d-3e874ae520d4","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-enz-mouse-high-fe-iron-sod2","predicate":"increases","statement":"After four weeks on the iron-enriched diet, nearly 80% of isolated mouse liver Sod2 was iron-loaded.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"f29d079d-15c4-558e-8ec9-9344238307ad","mechanism_event_label":"A high-iron mouse diet shifted Sod2 toward iron loading.","subject":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"object":{"id":"cd79f740-d578-5fb0-b3da-1fc30bc74a23","slug":"mouse-sod2-iron-loaded","display_name":"Iron-loaded mouse Sod2","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"f29d079d-15c4-558e-8ec9-9344238307ad","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-enz-mouse-high-fe-iron-sod2-event","event_type":"biochemical_relationship","label":"A high-iron mouse diet shifted Sod2 toward iron loading.","description":"After four weeks on the iron-enriched diet, nearly 80% of isolated mouse liver Sod2 was iron-loaded.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cd79f740-d578-5fb0-b3da-1fc30bc74a23","slug":"mouse-sod2-iron-loaded","display_name":"Iron-loaded mouse Sod2","entity_type_key":"protein_state"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"role":"normal Sod2 metal","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"role":"competing metal identity","stoichiometry":null,"state_label":"","sequence_order":3,"notes":"Element identity only; no fixed oxidation state assigned to total tissue iron or iron throughout the enzyme redox reaction."}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Iron excess altered occupancy of a Mn enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified SOD2, SOD2-overexpressing human cells, and dietary C57BL/6J mouse experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Five-week-old male C57BL/6J mice; 2700 ppm Fe and 150 ppm Mn versus control 275 ppm Fe and 150 ppm Mn, four weeks.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Experimental iron excess, not iron deficiency or a human oral-dose equivalence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Manganese research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"manganese","display_name":"Manganese","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A high-iron mouse diet shifted Sod2 toward iron loading.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mn-enz-29398562] Switch of Mitochondrial Superoxide Dismutase into a Prooxidant Peroxidase in Manganese-Deficient Cells and Mice. (2018). https://pubmed.ncbi.nlm.nih.gov/29398562/ DOI: 10.1016/j.chembiol.2018.01.007","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Liver Sod2","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"bd845a19-8152-543e-bb08-d4c1d3f82ae4","evidence_kind":"source_excerpt","locator":"Lines 521-532","start_line":521,"end_line":532,"excerpt":"### mn-enz-mouse-high-fe-iron-sod2\nAfter four weeks on the iron-enriched diet, nearly 80% of isolated mouse liver Sod2 was iron-loaded.\nCondition category: normal\nnutrient_topic: Manganese research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A high-iron mouse diet shifted Sod2 toward iron loading.\norganism: Mus musculus\ntissue_or_cell_type: Liver Sod2\nexperimental_model: Purified SOD2, SOD2-overexpressing human cells, and dietary C57BL/6J mouse experiments\nlimitations: Experimental iron excess, not iron deficiency or a human oral-dose equivalence.\nexposure: Five-week-old male C57BL/6J mice; 2700 ppm Fe and 150 ppm Mn versus control 275 ppm Fe and 150 ppm Mn, four weeks.\ncross_nutrient: Iron excess altered occupancy of a Mn enzyme.\n[mn-enz-29398562] Switch of Mitochondrial Superoxide Dismutase into a Prooxidant Peroxidase in Manganese-Deficient Cells and Mice. (2018). https://pubmed.ncbi.nlm.nih.gov/29398562/ DOI: 10.1016/j.chembiol.2018.01.007","model_system":"Purified SOD2, SOD2-overexpressing human cells, and dietary C57BL/6J mouse experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mn-enz-29398562] Switch of Mitochondrial Superoxide Dismutase into a Prooxidant Peroxidase in Manganese-Deficient Cells and Mice. 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