{"id":"07689450-cb5b-53b0-bf07-dba9ad977af1","stable_key":"7484635e-cc8f-5cd6-a978-6dc5775a7db5:berberine-abcb1-expression","predicate":"increases_expression_of","statement":"Berberine increased MDR1/ABCB1 expression; Nrf2 silencing abolished the gene response in Caco-2 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c174cbc0-821d-5039-a729-6a000c8ca217","mechanism_event_label":"Repeated exposure can increase the pump that exports drugs.","subject":{"id":"7bbe0633-b2f1-510f-adc0-3c4d0ddad05d","slug":"berberine","display_name":"Berberine","entity_type_key":"small_molecule"},"object":{"id":"c6139b52-4140-5622-9e33-1f6c6385265d","slug":"abcb1","display_name":"Human P-glycoprotein / ABCB1 / MDR1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"c174cbc0-821d-5039-a729-6a000c8ca217","stable_key":"7484635e-cc8f-5cd6-a978-6dc5775a7db5:berberine-abcb1-expression-event","event_type":"biochemical_relationship","label":"Repeated exposure can increase the pump that exports drugs.","description":"Berberine increased MDR1/ABCB1 expression; Nrf2 silencing abolished the gene response in Caco-2 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dc472dd3-d383-567a-ab37-a9ec4e112df4","slug":"nfe2l2","display_name":"Human Nrf2 / NFE2L2","entity_type_key":"protein"},"role":"required_transcription_factor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7bbe0633-b2f1-510f-adc0-3c4d0ddad05d","slug":"berberine","display_name":"Berberine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c6139b52-4140-5622-9e33-1f6c6385265d","slug":"abcb1","display_name":"Human P-glycoprotein / ABCB1 / MDR1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/berberine-research/29891588.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e59ba2b3b6e505cf3b220d32040e37f53553ea73427dd522cf7ea237d7d0f405\", \"start_char\": 0, \"end_char\": 1752, \"text_sha256\": \"e59ba2b3b6e505cf3b220d32040e37f53553ea73427dd522cf7ea237d7d0f405\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Nrf2 reporter, gene silencing and expression assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Berberine concentration and time response","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Transporter expression after prolonged exposure is not the same endpoint as acute competitive inhibition. Rat colitis improvement does not establish human IBD efficacy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Berberine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"berberine","display_name":"Berberine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human Caco-2 cells; rat colitis in a separate arm","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Repeated exposure can increase the pump that exports drugs.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[berberine-p29891588] Berberine Upregulates P-Glycoprotein in Human Caco-2 Cells and in an Experimental Model of Colitis in the Rat via Activation of Nrf2-Dependent Mechanisms. (2018). https://pubmed.ncbi.nlm.nih.gov/29891588/ DOI: 10.1124/jpet.118.249615","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Nrf2-dependent ABCB1 expression","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"586a97a2-051c-5e61-988c-7d15160620a4","evidence_kind":"source_excerpt","locator":"Lines 545-556","start_line":545,"end_line":556,"excerpt":"### berberine-abcb1-expression\nBerberine increased MDR1/ABCB1 expression; Nrf2 silencing abolished the gene response in Caco-2 cells.\nCondition category: normal\nnutrient_topic: Berberine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Repeated exposure can increase the pump that exports drugs.\norganism: Human Caco-2 cells; rat colitis in a separate arm\ntissue_or_cell_type: Nrf2-dependent ABCB1 expression\nexperimental_model: Nrf2 reporter, gene silencing and expression assays\nlimitations: Transporter expression after prolonged exposure is not the same endpoint as acute competitive inhibition. Rat colitis improvement does not establish human IBD efficacy.\nexposure: Berberine concentration and time response\nevidence_span: {\"source_cache\": \"artifacts/berberine-research/29891588.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e59ba2b3b6e505cf3b220d32040e37f53553ea73427dd522cf7ea237d7d0f405\", \"start_char\": 0, \"end_char\": 1752, \"text_sha256\": \"e59ba2b3b6e505cf3b220d32040e37f53553ea73427dd522cf7ea237d7d0f405\"}\n[berberine-p29891588] Berberine Upregulates P-Glycoprotein in Human Caco-2 Cells and in an Experimental Model of Colitis in the Rat via Activation of Nrf2-Dependent Mechanisms. (2018). https://pubmed.ncbi.nlm.nih.gov/29891588/ DOI: 10.1124/jpet.118.249615","model_system":"Nrf2 reporter, gene silencing and expression assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [berberine-p29891588] Berberine Upregulates P-Glycoprotein in Human Caco-2 Cells and in an Experimental Model of Colitis in the Rat via Activation of Nrf2-Dependent Mechanisms. (2018). https://pubmed.ncbi.nlm.nih.gov/29891588/ DOI: 10.1124/jpet.118.249615","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9bb61e1d-e5cf-5b05-a9ca-409291f7a313","stable_key":"import-7484635e-cc8f-5cd6-a978-6dc5775a7db5","title":"Berberine: metabolism, nutrient connections and drug interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"2f376a035ae79c93e62e2ea4a8d5385643bf16553f108d12b6a80c89e6d41f4c","revision_id":"2a099429-1c4a-55be-aa2c-0565d08bda80","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}