{"id":"07679ca1-1cb6-5af1-b1db-808fc5de4831","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-claudin-surface","predicate":"reduces_tested_surface_localization_of","statement":"The Caco-2 study found reduced cell-surface claudin 3 after 0.1 mM aspartame exposure.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5fe82011-e28c-5ed6-8253-be0e714eedfa","mechanism_event_label":"Junction location matters as well as protein presence.","subject":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"object":{"id":"2ccc9999-f1db-5fb7-b57c-513323c3e26e","slug":"cldn3","display_name":"Human claudin 3 / CLDN3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"5fe82011-e28c-5ed6-8253-be0e714eedfa","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-claudin-surface-event","event_type":"observed_relationship","label":"Junction location matters as well as protein presence.","description":"The Caco-2 study found reduced cell-surface claudin 3 after 0.1 mM aspartame exposure.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2ccc9999-f1db-5fb7-b57c-513323c3e26e","slug":"cldn3","display_name":"Human claudin 3 / CLDN3","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8f5d6b57-a2d6-51e9-aa10-0479162ac340","slug":"human-caco2-aspartame-permeability","display_name":"Human Caco-2 permeability during aspartame exposure","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text; Figure 4","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"24-hour exposure and surface ELISA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not establish direct aspartame–claudin binding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Junction location matters as well as protein presence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Artificial Sweeteners Disrupt Tight Junctions and Barrier Function in the Intestinal Epithelium through Activation of the Sweet Taste Receptor, T1R3. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32580504/ · DOI 10.3390/nu12061862","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"384fdf58-9fb8-51f3-b925-9a1cd4a7f857","evidence_kind":"source_excerpt","locator":"Lines 202-208","start_line":202,"end_line":208,"excerpt":"## aspartame-claudin-surface\nJunction location matters as well as protein presence.\nThe Caco-2 study found reduced cell-surface claudin 3 after 0.1 mM aspartame exposure.\nModel: 24-hour exposure and surface ELISA.\nLimitations: Does not establish direct aspartame–claudin binding.\nEvidence access: Primary full text; Figure 4\nArtificial Sweeteners Disrupt Tight Junctions and Barrier Function in the Intestinal Epithelium through Activation of the Sweet Taste Receptor, T1R3. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32580504/ · DOI 10.3390/nu12061862","model_system":"24-hour exposure and surface ELISA.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2bba7d36-993f-5ba7-acaf-2e4d573527ed","stable_key":"import-b428117d-3a8c-5e74-8711-168153e19545","title":"Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"21cfc6808062c6ea1fb5d34128bcd005d31f0f6c0d63484794238db77d32ae97","revision_id":"1255dda8-df99-5d4b-a65b-4144c47d7d9b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}