{"id":"07269f61-863e-5726-b32d-405d40a324e6","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-machinery","predicate":"required_in_model_for","statement":"Silencing MYPT1, eEF1A or 67LR abrogated EGCG-mediated tumor-growth inhibition in the tested models.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d4a8051e-1221-5bee-94af-c10030f3bdea","mechanism_event_label":"Removing pathway components prevented the observed response.","subject":{"id":"f3e7f45d-c517-54f4-bdf9-6628399b127b","slug":"mypt1-family","display_name":"Myosin phosphatase targeting subunit 1 family / MYPT1","entity_type_key":"protein_family"},"object":{"id":"f149fb2e-788c-564f-82ec-66cb1fb72789","slug":"egcg-model-tumor-growth","display_name":"Tumor growth in EGCG experimental models","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d4a8051e-1221-5bee-94af-c10030f3bdea","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-machinery-event","event_type":"observed_relationship","label":"Removing pathway components prevented the observed response.","description":"Silencing MYPT1, eEF1A or 67LR abrogated EGCG-mediated tumor-growth inhibition in the tested models.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f3e7f45d-c517-54f4-bdf9-6628399b127b","slug":"mypt1-family","display_name":"Myosin phosphatase targeting subunit 1 family / MYPT1","entity_type_key":"protein_family"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f149fb2e-788c-564f-82ec-66cb1fb72789","slug":"egcg-model-tumor-growth","display_name":"Tumor growth in EGCG experimental models","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"fd0edc10-cd26-5e13-af04-a8ab865108a4","slug":"eef1a-family","display_name":"Eukaryotic elongation factor 1A family","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"85e65054-3bd4-53b5-a5f0-2286061f8bf0","slug":"laminin-receptor-67kda","display_name":"67-kDa laminin receptor state / 67LR","entity_type_key":"protein_state"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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unverified.","entity":null}],"evidence":[{"id":"082bd810-7f29-5361-b56a-c9006865ad6f","evidence_kind":"source_excerpt","locator":"Lines 52-58","start_line":52,"end_line":58,"excerpt":"## egcg-machinery\nRemoving pathway components prevented the observed response.\nSilencing MYPT1, eEF1A or 67LR abrogated EGCG-mediated tumor-growth inhibition in the tested models.\nModel: Genetic screen, cell signaling and experimental tumor-growth models.\nLimitations: Pathway dependency in these models does not establish human cancer treatment or a dietary threshold.\nEvidence access: primary abstract.\nGreen tea polyphenol epigallocatechin-3-gallate signaling pathway through 67-kDa laminin receptor. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18079119/ · DOI 10.1074/jbc.m707892200","model_system":"Genetic screen, cell signaling and experimental tumor-growth models.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Primary-abstract paraphrase; no full-text methods verification claimed.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1f6d09e8-3aae-5c41-9ea7-15d0d1cab72d","stable_key":"import-ec987f3e-0b3c-55cc-b304-c703a738e35d","title":"EGCG: receptor signaling, metabolism, nutrient interactions and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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