{"id":"0703f5ca-f5f1-5ef2-9c84-fbacc4a0200b","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-ippk-ip6","predicate":"phosphorylates","statement":"Human IPPK used ATP to phosphorylate inositol 1,3,4,5,6-pentakisphosphate at position 2, producing InsP6.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"520762e5-b219-52c4-a381-8dddfa930953","mechanism_event_label":"The cell can make the six-phosphate inositol molecule inside itself.","subject":{"id":"096d32ee-898a-51e5-b941-6c133099ba94","slug":"ippk","display_name":"IPPK (human inositol-pentakisphosphate 2-kinase)","entity_type_key":"protein"},"object":{"id":"11d7be15-baab-5967-b198-5d99ed09114b","slug":"inositol-13456-pentakisphosphate","display_name":"Inositol 1,3,4,5,6-pentakisphosphate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"520762e5-b219-52c4-a381-8dddfa930953","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-ippk-ip6-event","event_type":"biochemical_relationship","label":"The cell can make the six-phosphate inositol molecule inside itself.","description":"Human IPPK used ATP to phosphorylate inositol 1,3,4,5,6-pentakisphosphate at position 2, producing InsP6.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f64ba327-66e7-52b6-9424-7d7ec11acca4","slug":"phytic-acid","display_name":"Phytic acid / phytate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"phosphate_donor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"096d32ee-898a-51e5-b941-6c133099ba94","slug":"ippk","display_name":"IPPK (human inositol-pentakisphosphate 2-kinase)","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"11d7be15-baab-5967-b198-5d99ed09114b","slug":"inositol-13456-pentakisphosphate","display_name":"Inositol 1,3,4,5,6-pentakisphosphate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/inositol-research/12084730.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7e44dbc7039048a53bffd92fd69dfa1f35f1dc762389c8d810f913ea70e10c80\", \"start_char\": 0, \"end_char\": 1407, \"text_sha256\": \"7e44dbc7039048a53bffd92fd69dfa1f35f1dc762389c8d810f913ea70e10c80\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant kinase and yeast complementation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Inositol pentakisphosphate and ATP assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Human enzyme activity; yeast rescue is not a clinical supplementation trial.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Inositol research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"inositol","display_name":"Inositol (stereoisomer family)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human IPPK expressed in Sf21 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The cell can make the six-phosphate inositol molecule inside itself.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ino-p12084730] The synthesis of inositol hexakisphosphate. Characterization of human inositol 1,3,4,5,6-pentakisphosphate 2-kinase. (2002). https://pubmed.ncbi.nlm.nih.gov/12084730/ DOI: 10.1074/jbc.m205682200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified kinase","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"80467cdf-03fe-5ef8-9964-3ea731b1c9dc","evidence_kind":"source_excerpt","locator":"Lines 743-754","start_line":743,"end_line":754,"excerpt":"### ino-ippk-ip6\nHuman IPPK used ATP to phosphorylate inositol 1,3,4,5,6-pentakisphosphate at position 2, producing InsP6.\nCondition category: normal\nnutrient_topic: Inositol research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The cell can make the six-phosphate inositol molecule inside itself.\norganism: Human IPPK expressed in Sf21 cells\ntissue_or_cell_type: Purified kinase\nexperimental_model: Recombinant kinase and yeast complementation\nlimitations: Human enzyme activity; yeast rescue is not a clinical supplementation trial.\nexposure: Inositol pentakisphosphate and ATP assays\nevidence_span: {\"source_cache\": \"artifacts/inositol-research/12084730.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7e44dbc7039048a53bffd92fd69dfa1f35f1dc762389c8d810f913ea70e10c80\", \"start_char\": 0, \"end_char\": 1407, \"text_sha256\": \"7e44dbc7039048a53bffd92fd69dfa1f35f1dc762389c8d810f913ea70e10c80\"}\n[ino-p12084730] The synthesis of inositol hexakisphosphate. Characterization of human inositol 1,3,4,5,6-pentakisphosphate 2-kinase. (2002). https://pubmed.ncbi.nlm.nih.gov/12084730/ DOI: 10.1074/jbc.m205682200","model_system":"Recombinant kinase and yeast complementation","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ino-p12084730] The synthesis of inositol hexakisphosphate. Characterization of human inositol 1,3,4,5,6-pentakisphosphate 2-kinase. (2002). https://pubmed.ncbi.nlm.nih.gov/12084730/ DOI: 10.1074/jbc.m205682200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d590c659-b774-50e6-9f1d-aff61d5f7c9c","stable_key":"import-e69ef6a0-0e23-5eac-aca1-cf333781b962","title":"Inositol: synthesis, signaling, mineral interactions and conditional deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f7adbcedad647d5f8a3cc9e46bfa515e83c508be9c2a4e9d59c5f5dfb0667d53","revision_id":"a82d187c-60fb-52d1-8482-eadb92860f33","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}