{"id":"06c0ca0b-418a-5d68-99d6-653d8758af85","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-extracellular-cystine-block","predicate":"inhibits_tested","statement":"Added extracellular glutamate inhibited cystine uptake in N18-RE-105 neuronal hybrid cells, and toxicity tracked that inhibition.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6f1a2ac1-c2b1-5c31-a1ee-1d043d4b7215","mechanism_event_label":"The same molecule used to build an antioxidant can obstruct another required ingredient when outside these cells.","subject":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"object":{"id":"4fdc07ad-8e1d-56e6-a03c-a8505891a79a","slug":"n18re105-cystine-uptake","display_name":"Cystine uptake in N18-RE-105 neuronal hybrid cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6f1a2ac1-c2b1-5c31-a1ee-1d043d4b7215","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-extracellular-cystine-block-event","event_type":"observed_relationship","label":"The same molecule used to build an antioxidant can obstruct another required ingredient when outside these cells.","description":"Added extracellular glutamate inhibited cystine uptake in N18-RE-105 neuronal hybrid cells, and toxicity tracked that inhibition.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4fdc07ad-8e1d-56e6-a03c-a8505891a79a","slug":"n18re105-cystine-uptake","display_name":"Cystine uptake in N18-RE-105 neuronal hybrid cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"cdd84f4d-3e8f-502a-a8c5-19672cc5346f","slug":"cystine","display_name":"Cystine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Neuroblastoma/primary-retina hybrid-cell culture; transport and toxicity assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The early paper does not genetically resolve SLC7A11; it is not an oral glutamate exposure study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The same molecule used to build an antioxidant can obstruct another required ingredient when outside these cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Glutamate toxicity in a neuronal cell line involves inhibition of cystine transport leading to oxidative stress. · 1989 · https://pubmed.ncbi.nlm.nih.gov/2576375/ · DOI 10.1016/0896-6273(89)90043-3","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3971963f-81c7-53da-9c33-1450811fde03","evidence_kind":"source_excerpt","locator":"Lines 330-336","start_line":330,"end_line":336,"excerpt":"## glutamate-extracellular-cystine-block\nThe same molecule used to build an antioxidant can obstruct another required ingredient when outside these cells.\nAdded extracellular glutamate inhibited cystine uptake in N18-RE-105 neuronal hybrid cells, and toxicity tracked that inhibition.\nModel: Neuroblastoma/primary-retina hybrid-cell culture; transport and toxicity assays.\nLimitations: The early paper does not genetically resolve SLC7A11; it is not an oral glutamate exposure study.\nEvidence access: Primary abstract\nGlutamate toxicity in a neuronal cell line involves inhibition of cystine transport leading to oxidative stress. · 1989 · https://pubmed.ncbi.nlm.nih.gov/2576375/ · DOI 10.1016/0896-6273(89)90043-3","model_system":"Neuroblastoma/primary-retina hybrid-cell culture; transport and toxicity assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c6a55239-e808-5ed7-9fa3-755a18ecb29f","stable_key":"import-b7014def-74ec-599e-8347-167e24fec1f0","title":"L-Glutamate / L-glutamic acid: carbon and nitrogen allocation, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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