{"id":"06434110-daa1-5844-9f51-21ebba6c34e1","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-hdc-external-aldimine","predicate":"binds","statement":"The human HDC inhibitor complex resolved a PLP-histidine-methyl-ester external aldimine in its active site.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"2693d6ae-4b87-5ccd-a0fa-837542ee1ecf","mechanism_event_label":"The structure shows how HDC holds a B6-bound substrate mimic.","subject":{"id":"fe5ba431-d43b-5aa2-8d4b-2cce24c1121c","slug":"hdc","display_name":"Human histidine decarboxylase / HDC","entity_type_key":"protein"},"object":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"2693d6ae-4b87-5ccd-a0fa-837542ee1ecf","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-hdc-external-aldimine-event","event_type":"biochemical_relationship","label":"The structure shows how HDC holds a B6-bound substrate mimic.","description":"The human HDC inhibitor complex resolved a PLP-histidine-methyl-ester external aldimine in its active site.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fe5ba431-d43b-5aa2-8d4b-2cce24c1121c","slug":"hdc","display_name":"Human histidine decarboxylase / HDC","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"988c0d59-a027-5cca-a035-bb42f5b6fb51","slug":"histidine-methyl-ester","display_name":"L-Histidine methyl ester","entity_type_key":"small_molecule"},"role":"inhibitor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Recombinant engineered human HDC; crystallography and enzyme assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Histidine methyl ester co-crystallization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Inhibitor-bound engineered protein; not direct observation of every physiological catalytic intermediate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The structure shows how HDC holds a B6-bound substrate mimic.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[komori-2012-hdc] Structural Study Reveals That Ser-354 Determines Substrate Specificity on Human Histidine Decarboxylase (2012). https://pmc.ncbi.nlm.nih.gov/articles/PMC3436558/ DOI: 10.1074/jbc.M112.381897","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant protein; no intact tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4aa96202-8962-5024-b1c0-cd23b741aa5b","evidence_kind":"source_excerpt","locator":"Lines 1059-1069","start_line":1059,"end_line":1069,"excerpt":"### b6-neuro-hdc-external-aldimine\nThe human HDC inhibitor complex resolved a PLP-histidine-methyl-ester external aldimine in its active site.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The structure shows how HDC holds a B6-bound substrate mimic.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant protein; no intact tissue\nexperimental_model: Recombinant engineered human HDC; crystallography and enzyme assays\nlimitations: Inhibitor-bound engineered protein; not direct observation of every physiological catalytic intermediate.\nexposure: Histidine methyl ester co-crystallization.\n[komori-2012-hdc] Structural Study Reveals That Ser-354 Determines Substrate Specificity on Human Histidine Decarboxylase (2012). https://pmc.ncbi.nlm.nih.gov/articles/PMC3436558/ DOI: 10.1074/jbc.M112.381897","model_system":"Recombinant engineered human HDC; crystallography and enzyme assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [komori-2012-hdc] Structural Study Reveals That Ser-354 Determines Substrate Specificity on Human Histidine Decarboxylase (2012). https://pmc.ncbi.nlm.nih.gov/articles/PMC3436558/ DOI: 10.1074/jbc.M112.381897","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}