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(2009). https://pubmed.ncbi.nlm.nih.gov/19017728/ DOI: 10.1152/ajpendo.90547.2008","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Kidney, liver, plasma and isolated hepatocytes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0d53e208-393f-5c09-9bbd-f1ca1131f1cc","evidence_kind":"source_excerpt","locator":"Lines 269-280","start_line":269,"end_line":280,"excerpt":"### creatine-hepatocyte-gaa-conversion\nIsolated rat hepatocytes synthesized creatine from guanidinoacetate but could not carry out the entire pathway from methionine, arginine and glycine under the tested conditions.\nCondition category: normal\nnutrient_topic: Creatine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: In this preparation, the liver needed the precursor supplied by the earlier synthesis step.\norganism: Rats\ntissue_or_cell_type: Kidney, liver, plasma and isolated hepatocytes\nexperimental_model: Rat feeding, isolated hepatocytes and in-vivo hepatic balance\nlimitations: Interorgan division of synthesis is established here in rats; this is not proof that human tissues never synthesize both steps locally.\nexposure: Creatine-fed versus creatine-free conditions; guanidinoacetate and methionine substrate experiments\nevidence_span: {\"source_cache\": \"artifacts/creatine-research/19017728.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"8394bd0e01991356b47a2e57593a5054e565b4020d50226b1331dbb290fc0cee\", \"start_char\": 0, \"end_char\": 1599, \"text_sha256\": \"8394bd0e01991356b47a2e57593a5054e565b4020d50226b1331dbb290fc0cee\"}\n[creatine-p19017728] Creatine synthesis: hepatic metabolism of guanidinoacetate and creatine in the rat in vitro and in vivo. 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