{"id":"05bed314-2a44-5d0b-8fb2-9d3268f59696","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:minus-lariciresinol-acts-on-transcription-not-rna-decay","predicate":"reduces","statement":"Promoter reporter assays and RNA decay dynamics indicated the hepatitis B virus RNA reduction was mainly transcriptional rather than a change in RNA stability.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"c2443194-225b-57c4-9112-433820e4e235","mechanism_event_label":"Promoter reporter assays and RNA decay dynamics indicated the hepatitis B virus RNA reduction was mainly transcriptional rather than a change in RNA stability.","subject":{"id":"bf9b95e9-f2d1-52bc-b68b-68f541085aeb","slug":"minus-lariciresinol","display_name":"(-)-Lariciresinol","entity_type_key":"small_molecule"},"object":{"id":"0fa79b73-dfc6-54af-9597-9b05a23f9e05","slug":"hbv-promoter-activity","display_name":"Hepatitis B virus promoter activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c2443194-225b-57c4-9112-433820e4e235","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:minus-lariciresinol-acts-on-transcription-not-rna-decay-event","event_type":"observed_relationship","label":"Promoter reporter assays and RNA decay dynamics indicated the hepatitis B virus RNA reduction was mainly transcriptional rather than a change in RNA stability.","description":"Promoter reporter assays and RNA decay dynamics indicated the hepatitis B virus RNA reduction was mainly transcriptional rather than a change in RNA stability.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bf9b95e9-f2d1-52bc-b68b-68f541085aeb","slug":"minus-lariciresinol","display_name":"(-)-Lariciresinol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0fa79b73-dfc6-54af-9597-9b05a23f9e05","slug":"hbv-promoter-activity","display_name":"Hepatitis B virus promoter activity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b6b0e047-df6c-5f77-a751-51019c928484","slug":"hbv-rna-production","display_name":"Hepatitis B virus RNA production","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Hepatoma cells with promoter luciferase reporters, with an RNA decay experiment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"(−)-Lariciresinol","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The two designs together separate transcription from decay; neither measures covalently closed circular DNA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Hepatoma cells with promoter luciferase reporters, with an RNA decay experiment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Promoter reporter assays and RNA decay dynamics indicated the hepatitis B virus RNA reduction was mainly transcriptional rather than a change in RNA stability.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Anti-Hepatitis B Virus Activity of (-)-Lariciresinol Isolated from the Roots of Isatis indigotica. (2022). https://pubmed.ncbi.nlm.nih.gov/35630700/ DOI: 10.3390/molecules27103180","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Viral promoter activity versus RNA decay rate","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f8b62b08-3e6c-5ca2-bc67-fc4ad8a6ac5d","evidence_kind":"source_excerpt","locator":"Lines 88-97","start_line":88,"end_line":97,"excerpt":"## minus-lariciresinol-acts-on-transcription-not-rna-decay\nPromoter reporter assays and RNA decay dynamics indicated the hepatitis B virus RNA reduction was mainly transcriptional rather than a change in RNA stability.\nModel/species: Hepatoma cells with promoter luciferase reporters, with an RNA decay experiment\nTissue/system: Viral promoter activity versus RNA decay rate\nExposure: (−)-Lariciresinol\nRoute: In vitro\nDuration: Not stated here\nLimits: The two designs together separate transcription from decay; neither measures covalently closed circular DNA.\nPrimary reference: Anti-Hepatitis B Virus Activity of (-)-Lariciresinol Isolated from the Roots of Isatis indigotica. (2022). https://pubmed.ncbi.nlm.nih.gov/35630700/ DOI: 10.3390/molecules27103180\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"48c58761-0291-5bad-a926-75e904b2d802","stable_key":"import-96d9034a-2550-5f3c-b767-1767c982533c","title":"Lariciresinol: five molecules under one name, and the mechanisms each one carries (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of twelve primary studies, every abstract read and all DOIs cross-checked against live PubMed metadata. Mechanism edges only, with no conclusion or claim of benefit recorded. Three author clusters account for eight of the twelve and carry shared laboratory keys. Study-specific concentrations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"6bf0015b7ff727b7a80d693dd18ffdd227d0e16ae45d9e85a5f81fa733edd3a4","revision_id":"219d9b0c-1a71-5e27-8d6c-7b3e77a594c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}