{"id":"05b8d04d-8e27-5fe5-9fa2-2a6718e03f33","stable_key":"33bef151-bdc7-5892-aff6-0345ab149739:nattokinase-cleaves-cross-linked-fibrin","predicate":"proteolyzes","statement":"Purified nattokinase cleaves cross-linked fibrin directly in a clot-lysis assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"e8bf5823-ef70-53f2-9804-2ec11ba297cc","mechanism_event_label":"Purified nattokinase cleaves cross-linked fibrin directly in a clot-lysis assay.","subject":{"id":"6b03a125-209a-5060-8b35-ec9e0e54033c","slug":"nattokinase","display_name":"Nattokinase / subtilisin NAT","entity_type_key":"peptide"},"object":{"id":"e74af504-cd81-586f-aa87-6af11978d478","slug":"fibrin","display_name":"Cross-linked fibrin","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"e8bf5823-ef70-53f2-9804-2ec11ba297cc","stable_key":"33bef151-bdc7-5892-aff6-0345ab149739:nattokinase-cleaves-cross-linked-fibrin-event","event_type":"biochemical_relationship","label":"Purified nattokinase cleaves cross-linked fibrin directly in a clot-lysis assay.","description":"Fibrin clot","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6b03a125-209a-5060-8b35-ec9e0e54033c","slug":"nattokinase","display_name":"Nattokinase / subtilisin NAT","entity_type_key":"peptide"},"role":"acting component","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e74af504-cd81-586f-aa87-6af11978d478","slug":"fibrin","display_name":"Cross-linked fibrin","entity_type_key":"protein"},"role":"component the finding is about","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Bibliographic record and the EFSA opinion's account of this study reviewed. The primary article itself was not retrieved and is not indexed in PubMed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human protein substrates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Purified nattokinase","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The primary report is in a journal PubMed does not index for this article and was not retrieved; the result is corroborated by the EFSA opinion, which cites the same work for the cross-linked fibrin finding. No validated preference map across fibrin alpha, beta, gamma and gamma-gamma chains under clinically relevant conditions was available in the sources read here.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human protein substrates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Purified nattokinase cleaves cross-linked fibrin directly in a clot-lysis assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[fujita-fibrin-1995] Characterization of nattokinase-degraded products from human fibrinogen or cross-linked fibrin. (1995) Not indexed in PubMed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Fibrin clot","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"05dd1fd2-5785-505a-8e1e-1ab7ba317ed5","evidence_kind":"source_excerpt","locator":"Lines 103-103","start_line":103,"end_line":103,"excerpt":"Purified nattokinase cleaves cross-linked fibrin directly in a clot-lysis assay.","model_system":"Human protein substrates","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"41cb127c-1c23-53aa-b7d0-5ec7f6fa3c99","stable_key":"import-33bef151-bdc7-5892-aff6-0345ab149739","title":"Nattokinase: what the purified enzyme cleaves, what survives being eaten, and the gap between the two (2026-09-23)","document_type":"imported_text","citation_label":"Original AI-assisted curation built from a supplied entity-first document of 105 entities and 129 claims. Every reference in that document was resolved against live PubMed with its abstract read and its DOI cross-checked on 2026-09-23, and the EFSA novel-food opinion was retrieved and read in full. That check corrected two PMIDs that pointed at unrelated papers, two DOIs, and two papers recorded as carrying no erratum that do carry one; it also reversed three findings the supplied document had stated backwards. Two papers carry a published correction, recorded as such and not as a retraction. Three sources are not indexed in PubMed and are cited by what they have. Laboratory lineages are recorded, so the four papers from one group, the three from another and the two readings of a single applicant dossier cannot be counted as separate lines of support. Study-specific doses, units, populations and limitations retained; activity units are never converted between systems. Not publisher full text.","file_path":"","sha256":"34fc46e0e849d06bfa13cb099a79b50679dd6fd0de8a5a0575326afdde3259c1","revision_id":"efd063e6-e982-5f6e-a4b8-6f2ffe4185ce","review_status":"unverified_draft","notes":""}}],"relations":[{"id":"b0260844-c251-516e-a5f8-de8e987274b3","relation_type":"limits_translation_of","notes":"Direct fibrin proteolysis requires the active enzyme to be present in plasma, and that has never been measured.","source_claim_id":"140e1014-8105-56ca-b13a-76fe0d42e8d9","source_claim_key":"33bef151-bdc7-5892-aff6-0345ab149739:efsa-could-not-conclude-on-absorption-of-the-active-enzyme","source_statement":"EFSA concluded that the information provided on absorption, distribution, metabolism and excretion did not allow conclusions to be drawn on the absorption of active nattokinase or any functional metabolites derived from it.","target_claim_id":"05b8d04d-8e27-5fe5-9fa2-2a6718e03f33","target_claim_key":"33bef151-bdc7-5892-aff6-0345ab149739:nattokinase-cleaves-cross-linked-fibrin","target_statement":"Purified nattokinase cleaves cross-linked fibrin directly in a clot-lysis assay."}],"conflicts":[],"corrections":[],"research":null}