{"id":"0595071c-af99-57b4-9379-9342af1cfdb1","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ver-dha-remodeling-flux","predicate":"modulates","statement":"Embryos from vitamin E-depleted zebrafish parents incorporated more water-18O label into LPC 22:6 and three DHA-containing phosphatidylcholines during 48–72 hours post-fertilization than vitamin E-sufficient controls, supporting increased acyl turnover.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e165af5e-c40a-5deb-a690-dbeaf9b0c692","mechanism_event_label":"Labeled-water tracing showed faster turnover of selected DHA lipids during vitamin E depletion.","subject":{"id":"40a6525a-76b8-576e-b73d-1e11aecf5f8d","slug":"alpha-tocopherol","display_name":"Alpha-tocopherol","entity_type_key":"small_molecule"},"object":{"id":"0c6c14f9-0929-5347-8596-276b443142d2","slug":"dha-phospholipid-remodeling","display_name":"DHA phospholipid remodeling","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e165af5e-c40a-5deb-a690-dbeaf9b0c692","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ver-dha-remodeling-flux-event","event_type":"biochemical_relationship","label":"Labeled-water tracing showed faster turnover of selected DHA lipids during vitamin E depletion.","description":"Embryos from vitamin E-depleted zebrafish parents incorporated more water-18O label into LPC 22:6 and three DHA-containing phosphatidylcholines during 48–72 hours post-fertilization than vitamin E-sufficient controls, supporting increased acyl turnover.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4800975a-b8bb-54ba-ae20-8f709ddefe78","slug":"lpc-22-6","display_name":"Lysophosphatidylcholine 22:6","entity_type_key":"lipid"},"role":"labeled DHA lysophospholipid","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"033a2800-fb71-5405-a144-a8e225bf456b","slug":"pc-16-0-22-6","display_name":"PC(16:0/22:6)","entity_type_key":"lipid"},"role":"one labeled DHA phospholipid","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6a008748-55ad-5eef-9ca2-73c14b7fe4a3","slug":"rrr-alpha-tocopheryl-acetate","display_name":"RRR-alpha-tocopheryl acetate","entity_type_key":"small_molecule"},"role":"parental dietary form","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"40a6525a-76b8-576e-b73d-1e11aecf5f8d","slug":"alpha-tocopherol","display_name":"Alpha-tocopherol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"0c6c14f9-0929-5347-8596-276b443142d2","slug":"dha-phospholipid-remodeling","display_name":"DHA phospholipid remodeling","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Figure 7; isotope-labeling Methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Parental diet manipulation and stable-isotope lipidomics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Parents fed no added E or 500 mg RRR-alpha-tocopheryl acetate/kg for ≥80 days; embryos incubated in 40% H2(18)O from 48 to 72 hpf.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Embryos rely on maternally deposited nutrients; whole-embryo tracing does not directly measure brain delivery or prove a particular repair enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin E research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-e","display_name":"Vitamin E","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Danio rerio","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Labeled-water tracing showed faster turnover of selected DHA lipids during vitamin E depletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ver-mcdougall2016] Lipidomics and H2(18)O labeling techniques reveal increased remodeling of DHA-containing membrane phospholipids associated with abnormal locomotor responses in α-tocopherol deficient zebrafish (danio rerio) embryos. (2016). https://pubmed.ncbi.nlm.nih.gov/26774753/ DOI: 10.1016/j.redox.2016.01.004","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Whole embryos","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"4f5236a3-22f3-5b93-acc1-2a419a64cde3","evidence_kind":"source_excerpt","locator":"Lines 760-772","start_line":760,"end_line":772,"excerpt":"### ver-dha-remodeling-flux\nEmbryos from vitamin E-depleted zebrafish parents incorporated more water-18O label into LPC 22:6 and three DHA-containing phosphatidylcholines during 48–72 hours post-fertilization than vitamin E-sufficient controls, supporting increased acyl turnover.\nCondition category: nutrient_deficiency\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Labeled-water tracing showed faster turnover of selected DHA lipids during vitamin E depletion.\norganism: Danio rerio\ntissue_or_cell_type: Whole embryos\nexperimental_model: Parental diet manipulation and stable-isotope lipidomics\nlimitations: Embryos rely on maternally deposited nutrients; whole-embryo tracing does not directly measure brain delivery or prove a particular repair enzyme.\nexposure: Parents fed no added E or 500 mg RRR-alpha-tocopheryl acetate/kg for ≥80 days; embryos incubated in 40% H2(18)O from 48 to 72 hpf.\ncross_nutrient: true\nevidence_location: Figure 7; isotope-labeling Methods\n[ver-mcdougall2016] Lipidomics and H2(18)O labeling techniques reveal increased remodeling of DHA-containing membrane phospholipids associated with abnormal locomotor responses in α-tocopherol deficient zebrafish (danio rerio) embryos. (2016). https://pubmed.ncbi.nlm.nih.gov/26774753/ DOI: 10.1016/j.redox.2016.01.004","model_system":"Parental diet manipulation and stable-isotope lipidomics","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ver-mcdougall2016] Lipidomics and H2(18)O labeling techniques reveal increased remodeling of DHA-containing membrane phospholipids associated with abnormal locomotor responses in α-tocopherol deficient zebrafish (danio rerio) embryos. 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