{"id":"057f2342-2df3-5318-9270-70cc04edbca4","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-acmsd-inhibition-nad","predicate":"inhibition_increases","statement":"ACMSD inhibition increased de novo NAD synthesis and SIRT1-related mitochondrial function in the mouse experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a260dd4f-bef1-5ba0-8ff6-f620d0881aa8","mechanism_event_label":"Restricting one exit route increased flow toward NAD in a preclinical model.","subject":{"id":"ddf5fa30-48dc-51c4-ac0f-98fe1a34e178","slug":"mouse-acmsd","display_name":"Mouse ACMS decarboxylase / Acmsd","entity_type_key":"protein"},"object":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"a260dd4f-bef1-5ba0-8ff6-f620d0881aa8","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-acmsd-inhibition-nad-event","event_type":"observed_relationship","label":"Restricting one exit route increased flow toward NAD in a preclinical model.","description":"ACMSD inhibition increased de novo NAD synthesis and SIRT1-related mitochondrial function in the mouse experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ddf5fa30-48dc-51c4-ac0f-98fe1a34e178","slug":"mouse-acmsd","display_name":"Mouse ACMS decarboxylase / Acmsd","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"dbe09a67-bc1f-53d9-b555-580c204b2bbb","slug":"mouse-sirt1","display_name":"Mouse sirtuin 1 / Sirt1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ab444b3c-c8ec-5ada-8450-36ac9934d6b6","slug":"quinolinic-acid","display_name":"Quinolinic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse genetic/pharmacological work within a study also using C. elegans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not a human longevity outcome or evidence for tryptophan megadoses.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; 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